Molecular cloning and expression of an IL-6 signal transducer, gp130

doi:10.1016/0092-8674(90)90411-7

Cell

Volume 63, Issue 6, 21 December 1990, Pages 1149-1157

https://doi.org/10.1016/0092-8674(90)90411-7 Get rights and content

Abstract

Interleukin-6 (IL-6) signal is transduced through a membrane glycoprotein, gp130, which associates with IL-6 receptor (IL-6-R). A cDNA encoding human gp130 has been cloned, revealing that it consists of 918 amino acids with a single transmembrane domain. The extracellular region comprises six units of a fibronectin type III module, and part of this region of ∼200 amino acids has features typical of a cytokine receptor family. A cDNA-expressed gp130 showed no binding property to IL-6 or several other cytokines. Although a transfectant with an IL-6-R cDNA expressed mainly low affinity IL-6 binding sites, an increase in high affinity binding sites was observed after cotransfection with a gp130 cDNA. This confirmed that a gp130 is involved in the formation of high affinity IL-6 binding sites. A cloned gp130 could associate with a complex of IL-6 and soluble IL-6-R and transduce the growth signal when expressed in a murine IL-3—dependent cell line.

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Cell

ArticleMolecular cloning and expression of an IL-6 signal transducer, gp130

Abstract

FEBS Lett.

Biochem. Biophys. Res. Commun.

Mol. Immunol.

Cell

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J. Biol. Chem.

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Blood

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Blood

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A nuclear factor for IL-6 expression (NF-IL6) is a member of a CEBP family

EMBO J.

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Article
Molecular cloning and expression of an IL-6 signal transducer, gp130

A nuclear factor for IL-6 expression (NF-IL6) is a member of a $C EBP$ family