Research reportMolecular cloning and characterization of cDNA encoding tryptophan hydroxylase from rat central serotonergic neurons
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Cited by (70)
Tryptophan hydroxylase and serotonin synthesis regulation
2020, Handbook of Behavioral NeuroscienceCitation Excerpt :Therefore, the molecular properties of TPH from crude rodent midbrain extracts are in remarkable agreement with the apparent molecular weight of 224–236 kDa calculated from the amino acid sequence of the cloned protein (Carkaci-Salli et al., 2006; Sakowski, Geddes, & Kuhn, 2006a; Walther et al., 2003). It was generally accepted that TPH was a single gene product, like the other members of the monooxygenase family of enzymes, so the differences in TPH behavior was initially attributed to differences in tissue-specific expression, differential translational efficiency (Dumas, Darmon, Delort, & Mallet, 1989), or even posttranslational events (Kim et al., 1991; Kuhn, Meyer, & Lovenberg, 1980). The earliest TPH cloning attempts used pineal gland (Darmon, Grima, Cash, Maitre, & Mallet, 1986; Grenett, Ledley, Reed, & Woo, 1987; Kim et al., 1991), a human carcinoid tumor (Boularand, Darmon, Ganem, Launay, & Mallet, 1990) or a murine mastocytoma (Stoll & Goldman, 1991), and this may have unwittingly added to the differences observed in the physical properties of TPH from tissue to tissue.
Life without brain serotonin: Reevaluation of serotonin function with mice deficient in brain serotonin synthesis
2015, Behavioural Brain ResearchCitation Excerpt :All aromatic amino acid hydroxylases are composed of three functional domains, a regulatory N-terminal domain, a catalytic domain, and a C-terminal oligomerization domain [4,5]. Since the discovery of the 5-HT synthesis pathway, the existence of only one gene coding for a TPH enzyme in vertebrates has been supposed [6]. However, in 2003 the existence of a second gene, Tph2, was unraveled by genetic ablation of the by that time only known Tph gene, now called Tph1 [7,8].
Gender differences in tryptophan hydroxylase-2 mRNA, serotonin, and 5-hydroxytryptophan levels in the brain of catfish, Clarias gariepinus, during sex differentiation
2011, General and Comparative EndocrinologyCitation Excerpt :The second form, tph2, is neuron-specific and expressed primarily in serotonergic neurons of the mice brain [37]. Both forms of tph have subsequently been cloned from other vertebrate species, such as human, mouse, rat, and zebrafish [1,2,15,32]. In brain, 5-HT acts as a neurotransmitter and also regulates the secretion of various reproductive neuroendocrine hormones and sexual behavior in vertebrates [10,13,14,24].
Dimorphic expression of tryptophan hydroxylase in the brain of XX and XY Nile tilapia during early development
2010, General and Comparative EndocrinologyGenetic Organization of the Serotonergic System
2010, Handbook of Behavioral NeuroscienceTryptophan Hydroxylase and Serotonin Synthesis Regulation
2010, Handbook of Behavioral NeuroscienceCitation Excerpt :TPH cDNA was first cloned in 1987 from a rabbit peripheral organ, the pineal gland (Grenett et al., 1987). Since then, many cDNAs have been cloned from various libraries of other sources including the brain (Darmon et al., 1988; Boularand et al., 1990; Stoll et al., 1990; Kim et al., 1991; D'Sa et al., 1996; Florez et al., 1996; Kowlessur and Kaufman, 1999). The full-length amino acid sequence of TPH has not been determined with purified enzyme, and hence has never been compared with the sequence deduced from the cDNA.