Positional dependence of enterocyte membrane potential in hamster and rabbit enterocytes

doi:10.1016/0300-9629(84)90613-3

Comparative Biochemistry and Physiology Part A: Physiology

Volume 78, Issue 4, 1984, Pages 661-666

Comparative Biochemi...

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Abstract

1.
1. A technique is described allowing microelectrode impalement of enterocytes located at known positions along intestinal villi from rabbits and hamsters. Using this technique a 5 mV hyperpolarization in membrane potential is shown to occur as enterocytes migrate over the basal third of intestinal villi.
2.
2. The villus structure of the hamster ileum is similar to the rabbit, but the enterocyte lifespan in these two tissues differs considerably (enterocyte migration rates of 17.6 and 6.3 μm hr⁻¹ for hamster and rabbit respectively).
3.
3. A correlation was found between the position an enterocyte occupied on the crypt-villus axis and the developmental state of the membrane potential. No such correlation existed when making comparisons on a time basis.
4.
4. These results are discussed both in terms of what is now known concerning different aspects of enterocyte development and in relation to what type of control mechanism might be generally responsible for initiating differentiation in this tissue.

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A preparation of intestinal villi suitable for recording membrane potentials in enterocytes taken at different stages of differentiation

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Glucose depolarizes villous but not crypt cell apical membrane potential difference: a micropuncture study of crypt-villus heterogeneity in the rat
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Brush-border membrane potentials and fractional resistances have been recorded from enterocytes at different points along the crypt-villus axis of rat ileum in vitro. Microelectrode impalements were obtained under visual control and brush-border membrane potentials were higher in crypt than in villous cells (−57 ± 1.6 against −50 ± 1.6 mV referred to the mucosal side). Replacing mannitol with d-glucose in the mucosal perfusate resulted in a rise in transmural potential difference (0.5 ± 0.17 to 1.0 ± 0.21 mV (n = 37)) and apical membrane potential was depolarized. This occurred consistently only in the upper two-thirds of the villus (−54 ± 1.7 to −47 ± 2.3 mV (n = 17)) and not in crypt cells (−56 ± 2.6 to −57 ± 2.4 mV (n = 10) or at the crypt-villus junction. The glucose-induced apical membrane depolarization in villous enterocytes was blocked by phlorizin, a competitive inhibitor of sodium-dependent glucose uptake (−50 ± 2.1 to −53 ± 2.8 mV (n = 9) in the presence of phlorizin and glucose). Transmural resistance, R_t, and fractional resistance, F_R, were unaltered by glucose ( $61 ± 3.4 to 61 ± 3.5 Ω· cm^{2} (n = 50)$ ) and (0.60 ± 0.06 to 0.57 ± 0.06 (n = 17)). This micro-puncture technique provides dirct evidence for functional differentiation along the crypt-villus axis and indicates that active electrogenic accumulation of glucose is confined to villous epithelium.
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Comparative Biochemistry and Physiology Part A: Physiology

Abstract

References (25)

Energetics of coupled Na⁺ and Cl⁻ entry into epithelial cells of bullfrog small intestine

Biochim. biophys. Acta

Membrane potentials of differentiating enterocytes

Biochim. biophys. Acta

A comprehensive description of brush border membrane development applying to enterocytes taken from a large variety of mammalian species

Comp. Biochem. Physiol.

Membrane potential changes associated with differentiation of enterocytes in the rat intestinal villi in culture

Devl Biol.

Effects of starvation and hyperglucagonaemia on the potential difference across the brush border membrane of rat small intestine

Gastroenterol. clin. Biol.

Intracellular chloride activities and active chloride absorption in the intestinal epithelium of the winter flounder

J. Membr. Biol.

Sodium-coupled amino acid and sugar transport by Necturus small intestine. An equivalent electrical circuit analysis of a rheogenic co-transport system

J. Membr. Biol.

A quantitative histochemical study of dipeptidylpeptidase IV (DPP IV)

Histochemistry

A quantitative histochemical technique for the characterisation of α-glucosidases in the brush border membrane of rat jejunum

Histochemistry

Transcellular ion route in rabbit gall-bladder. Electric properties of the epithelial cells

Pflügers Arch.

Inductive processes in embryonic development

Science

A preparation of intestinal villi suitable for recording membrane potentials in enterocytes taken at different stages of differentiation

J. Physiol. (Lond.)

Cited by (6)

Glucose depolarizes villous but not crypt cell apical membrane potential difference: a micropuncture study of crypt-villus heterogeneity in the rat

The second brain in autism spectrum disorder: Could connexin 43 expressed in enteric glial cells play a role?

Meta-analysis of the turnover of intestinal epithelia in preclinical animal species and humans

Meta-analysis of the turnover of intestinal epithelia in preclinical animal species and humans

Intestinal transport in megacolonic mice - Alterations in sugar absorption

Starvation-induced changes in the autoradiographic localisation of valine uptake by rat small intestine