Short communicationLocalization of a phenobarbital-responsive element (PBRE) in the 5′-flanking region of the rat CYP2B2 gene
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2016, Acta Pharmaceutica Sinica BInsights into CYP2B6-mediated drug-drug interactions
2016, Acta Pharmaceutica Sinica BCitation Excerpt :Over the years, researchers have observed potent induction of CYP2B genes by barbiturates in the liver of many different species. The first study illustrating molecular mechanisms behind this induction came in 1995 using cultured adult rat hepatocytes124. A functional analysis of the 5ʹ flanking promoter region of rat Cyp2b1 and Cyp2b2 has linked PB-mediated induction to a 163-bp DNA sequence, termed the PB-responsive element (PBRE) or PB-responsive unit (PBRU), located −2155 to 2318 bp from the transcription start site of Cyp2b1/2124,125.
Induction of a detoxification gene in Drosophila melanogaster requires an interaction between tissue specific enhancers and a novel cis-regulatory element
2011, Insect Biochemistry and Molecular BiologyCitation Excerpt :The barbiturate compound Phenobarbital (PB) has been extensively used to study the induction mechanism of mammalian P450 genes. Reporter gene assays in cultured adult rat hepatocytes were used to identify a 163 bp PB responsive element (PBRE) in the regulatory region of the rat P450 gene CYP2B2 (Trottier et al., 1995). This PBRE was subsequently shown to be active in adult rat liver (Park et al., 1996).
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2011, Biochimica et Biophysica Acta - Proteins and ProteomicsWerner's syndrome helicase participates in transcription of phenobarbital-inducible CYP2B genes in rat and mouse liver
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