Assay of 6-thioinosinic acid and 6-thioguanine nucleotides, active metabolites of 6-mercaptopurine, in human red blood cells
References (23)
- et al.
Anal. Biochem.
(1985) - et al.
Lancet
(1986) - et al.
J. Chromatogr.
(1973) - et al.
J. Chromatogr.
(1978) - et al.
Anal. Biochem.
(1987) - et al.
J. Chromatogr.
(1982) - et al.
Biochem. Pharmacol.
(1975) J. Chromatogr.
(1985)Cancer Res.
(1963)- et al.
J. Pharm. Pharmacol.
(1983)
Cancer Res.
Cited by (92)
Determination of azathioprine/6-mercaptopurine metabolites in dried blood spots: Correlation with RBC concentrations
2020, Journal of Pharmaceutical and Biomedical AnalysisCitation Excerpt :The analysis of AZA/6-MP metabolites in biological samples is not straight forward and requires pre-treatment of samples. Several studies have used the Lennard methodology [15,16] to measure 6-TG in TDM applications. The Lennard method is based on the hydrolysis of 6-thioguanine mono-, di- and triphosphates into the free base 6-TG which are then measured as total thioguanine nucleotides (6-TGNs).
Antineoplastic drugs
2020, Toxicology Cases for the Clinical and Forensic LaboratoryMicrobial metabolism of thiopurines: A method to measure thioguanine nucleotides
2016, Journal of Microbiological MethodsCitation Excerpt :In addition, there is lack of commercially available isotopic calibrators to detect TGNs directly as mono-, di- and triphosphates (Keuzenkamp-Jansen et al., 1995, Kirchherr et al., 2013). The existing commercial assays are largely based on acid hydrolysis of TGNs to convert the thio-nucleotides to thiopurine base, although nucleobases, nucleosides and nucleotides cannot be distinguished by these approaches (Dervieux et al., 2002, Lennard, 1987). An alternative approach uses enzymatic hydrolysis to convert TGNs to thiopurine nucleoside (thioguanine riboside: TGr) (Dervieux et al., 2002), but this is not used commercially.
Consensus guidelines of ECCO/ESPGHAN on the medical management of pediatric Crohn's disease
2014, Journal of Crohn's and ColitisDetermination of 6-thioguanine based on localized surface plasmon resonance of gold nanoparticle
2013, Spectrochimica Acta - Part A: Molecular and Biomolecular SpectroscopyCitation Excerpt :The anticancer drug 6-thioguanine (6-TG) is one kind of purine compound and used to influence immune response. Some methods have been reported for the determination of 6-TG, including voltammetry [13], fluorimetry [14], luminescence analysis [15], HPLC [16–20], and electrochemical method [21]. However, there are still some limits in these methods.
Improved HPLC Quantification of 6-Mercaptopurine Metabolites in Red Blood Cells: Monitoring Data and Literature Analysis
2022, International Journal of Molecular Sciences