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Determination of berberine in plasma, urine and bile by high-performance liquid chromatography

https://doi.org/10.1016/0378-4347(94)00517-9Get rights and content

Abstract

A high-performance liquid chromatographic method for the determination of berberine in plasma, urine and bile samples is described. Plasma samples were pretreated by protein precipitation with acetonitrile and urine and bile samples were pretreated by organic solvent extraction using 5% 2-propanol in methylene chloride. Berberine was determined in all samples using an octyl reversed-phase column with a mobile phase of 60–63% acetonitrile in 0.1% phosphoric acid (pH 6.0) and with UV detection at 267 nm. The detection limits for berberine in plasma, urine and bile were 18.1 ng/ml, 2.3 ng/ml and 90.4 ng/ml, respectively. The recoveries of berberine by simple deproteinization of plasma and by solvent extraction of urine were 78.3 and 82.9%, respectively. The intra-day and inter-day accuracy and precision for plasma reported as coefficients of variation and relative errors were both less then 6%. The applicability of the assay to pharmacokinetic and bioavailability studies was demonstrated by the determination of berberine in plasma, urine and bile after intravenous and intramuscular administration to rabbits at a dose of 2 mg/kg.

References (11)

  • H. Miyazaki et al.

    J. Chromatogr.

    (1978)
  • G.D. Pandey et al.

    Heterocycles

    (1980)
  • K. Takagi

    Pharmacology of Medicinal Herbs in East Asia

  • M. Sabir et al.

    Indian J. Physiol. Pharmacol.

    (1971)
There are more references available in the full text version of this article.

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    Citation Excerpt :

    Therefore, developing a sensitive method for determination of Brb in plasma and urine is inevitable for evaluating the pharmacokinetics of Brb in human [25]. Despite the reported methods for determination of Brb in biological samples taken from rat, mice and rabbit [26–30], there are only few reports dealing with its determination in human plasma and urine samples by different instrumental methods such as LC-ESI-MS [31], capillary zone electrophoresis (CZE) [32], high performance liquid chromatography with UV detection (HPLC-UV) [33,34], and gas chromatography-MS (GC–MS) [35]. Due to the matrix complexity in biofluid samples and with regard to the trace amounts of Brb in human plasma, utilization of an extraction technique prior to instrumental analysis is suitable to improve the detection results by simultaneous sample clean-up and analyte enrichment.

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