Determination of dicentrine in rat plasma by high-performance liquid chromatography and its application to pharmacokinetics

doi:10.1016/0378-4347(95)00553-6

Journal of Chromatography B: Biomedical Sciences and Applications

Volume 681, Issue 2, 7 June 1996, Pages 277-281

https://doi.org/10.1016/0378-4347(95)00553-6 Get rights and content

Abstract

A simple high-performance liquid chromatographic method was developed to study the pharmacokinetics of dicentrine in rat plasma after 10 mg/kg intravenous administration. After addition of an internal standard (coumarin), plasma was deproteinized by acetonitrile for sample clean-up. The drugs were separated on a reversed-phase Nucleosil C₁₈ column (250 × 4 mm I.D., particle size 5 μm) and detected by photodiode-array detection at a wavelength of 308 nm. Acetonitrile-water (35:65, v/v, pH 2.5–2.8, adjusted with orthophosphoric acid) was used as the mobile phase. A biphasic phenomenon with a rapid distribution followed by a slower elimination phase was observed from the plasma concentration-time curve.

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It confirms the traditional use of this plant as an antihypertensive. Lai et al. [8] and Tsai et al. [9] proposed two HPLC methods for the quantification of dicentrine in rat plasma and urine. The first method was not validated [8].
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Regular paperDetermination of dicentrine in rat plasma by high-performance liquid chromatography and its application to pharmacokinetics

Abstract

J. Pharm. Sci.

J. Pharm. Sci.

J. Chromatogr. B.

Planta Med.

Br. J. Pharmacol.

Regular paper
Determination of dicentrine in rat plasma by high-performance liquid chromatography and its application to pharmacokinetics