Original article: general thoracicSingle-pass isolated lung perfusion versus recirculating isolated lung perfusion with melphalan in a rat model
Section snippets
Animals
Male inbred WAG-Rij strain rats (approximately 200 g), obtained from Harlan-CPB (Zeist, The Netherlands), were used for all experiments. Animals were treated in accordance with the Animal Welfare Act and the “Guide for the Care and Use of Labaratory Animals” (National Institutes of Health Publication 86 to 23, revised 1985). The rats were transported in sterile conditions, housed in suspended mesh wired cages, and ad libitum fed a standard pellet diet (standard rat chow; Hope Farms, Woerden,
Mortality
All rats survived injection of tumor cells, and the procedure-related survival was 100% for all experiments.
Experiment 1
The temperature of the reservoir and the rats was not significantly different between groups (Table 1). Hematocrit levels were significantly lower for groups 1 and 2 (BHE perfusate) compared with groups 3 and 4 (RBC-BHE perfusate). No significant difference was seen in wet-to-dry ratios between the groups (Table 1).
No significant difference was seen in the left lung MN levels of rats
Comment
Whereas the lungs are the most common site of metastases [14], it is known that for sarcoma and carcinoma metastases, up to 50% of the patients have recurrent disease exclusively in the lung after resection of the primary disease, probably due to the presence of micrometastases at the time of the initial operation 1, 15. Recurrent disease can be inoperable due to size, number, or location of these lung metastases. Therefore, it is important to have local control of microscopic disease after
Acknowledgements
This work was supported by a research grant from the University of Antwerp.
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Regional Chemotherapy for Carcinoma of the Lung
2008, Surgical Oncology Clinics of North AmericaCitation Excerpt :By means of these inserted catheters, an isolated circuit of the thoracic region can be established for 30 minutes without the need for an additional pumping system. Pharmacokinetic evaluations in different mouse models have shown that using thoracic and lung perfusion techniques, cytostatic drug concentrations of melphalan, doxorubicin, cisplatin, 5-fluorouracil, and mitomycin are 6 to 10 times higher compared with systemic administration [7–10]. The technique of isolated perfusion was established by Creech and colleagues [4] in 1958 when they performed an isolated extremity perfusion.
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