Methods for routine biological monitoring of carcinogenic PAH-mixtures

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Abstract

The ability of a biomarker to provide an assessment of the integrated individual dose following uptake through multiple routes is especially valuable for mixtures of polycyclic aromatic hydrocarbons (PAH), due to methodological and practical difficulties of collecting and analysing samples from the various environmental compartments like air, water and soil and various media such as diet, cigarette smoke and workroom air. Since 1980, a large variety of novel approaches and techniques have been suggested and tested, e.g. urinary thioethers, mutagenicity in urine, levels of PAH or PAH-metabolites in blood and urine and methods for determination of adducts in DNA and proteins. Two approaches are more frequently reported: PAH-DNA-adduct monitoring in blood cells and urinary 1-hydroxypyrene monitoring. A large research effort has been made to use the extent of binding of PAH to DNA as a biomarker of exposure. The 32P-post-labeling assay detects the total of aromatic DNA-adducts and the adduct level in white blood cells is claimed to be an indicator of the biological effect of the PAH-mixture. However, the levels of aromatic DNA-adducts may be subject to appreciable analytical and biological variation. The present technical complexity of the method makes it more convenient for research applications than for routine application in occupational health practice. Pyrene is a dominant compound in the PAH mixture and is mainly metabolised to the intermediary 1-hydroxypyrene to form 1-hydroxypyrene-glucuronide, which is excreted in urine. Since the introduction of the determination of 1-hydroxypyrene in urine as a biomarker for human exposure assessment in 1985, many reports from different countries from Europe, Asia and America confirmed the potential of this novel approach. The conclusion of the first international workshop on 1-hydroxypyrene in 1993 was that urinary 1-hydroxypyrene is a solid biological exposure indicator of PAH. Studies with a comparison of several biomarkers confirmed that 1-hydroxypyrene in urine is a valid and sensitive indicator of exposure. Periodical monitoring of 1-hydroxypyrene appears to be a powerful method in controlling occupational PAH-exposure in industries. The reference level and the biological exposure limit of 1-hydroxypyrene in urine are discussed.

References (57)

  • Z. Zhao et al.

    Experiments on the effects on the 1-hydroxypyrene level in human urino as an indicator of exposure to PAH

    Sci. Total Environ.

    (1992)
  • F. Ariese

    Shpolskii spectroscopy: biomonitoring of PAH and their metabolites

  • A.C. Beach et al.

    Human biomonitoring and 32P-postlabeling assay

    Carcinogenesis

    (1992)
  • G. Becher et al.

    Multimethod determination of occupational exposure to PAH in an aluminum plant

    Carcinogenesis

    (1984)
  • P.J. Boogaard et al.

    Measurement of exposure to PAH in petrochemical industries by determination of urinary 1-hydroxypyrene

    Occup. Environ. Med.

    (1994)
  • M. Bouchard et al.

    Improved procedure for HPLC determination of monohydroxylated PAH-metabolites in urine

    J. Anal. Toxicol.

    (1994)
  • T.J. Buckley et al.

    An examination of the time course from human dietary exposure to PAH to urinary elimiantion of 1-hydroxypyrene

    Br. J. Ind. Med.

    (1992)
  • T.J. Buckley et al.

    An assessment of a urinary biomarker for total human environmental exposure to benzo(a)pyrene

    Int. Arch. Occup. Environ. Health

    (1995)
  • J.P. Buchet et al.

    Evaluation of exposure to PAH in a coke production and graphite electrode manufacturing plant

    Br. J. Ind. Med.

    (1992)
  • J.P. Buchet et al.

    Tumor markers in serum, polyamines and modified nucleosides in urine, and cytogenetic aberrations in lymphocytes of workers exposed to PAH

    Am. J. Ind. Med.

    (1995)
  • S. Burgaz et al.

    Evaluation of urinary 1-hydroxypyrene and thioethers in workers exposed to bitumen fumes

    Int. Arch. Occup. Environ. Health

    (1992)
  • E. Clonfero et al.

    Biological monitoring of human exposure to coal tar. Urinary mutagenicity assay and analytical determination of PAH-metabolites in urine

  • E. Clonfero et al.

    Biological monitoring of human exposure to coal tar. Urinary excretion of total polycyclic aromatic hydrocarbons, 1-hydroxypyrene and mutagens in psoriatic patients

    Int. Arch. Occup. Environ. Health

    (1989)
  • M. Dell'Omo et al.

    Adducts to macromolecules in the biological monitoring of PAH

    Crit. Rev. Toxicol.

    (1993)
  • E. Elovaara et al.

    Significance of dermal and respiratory uptake in creosote workers; exposure to PAH and urinary excretion of 1-hydroxypyrene

    Occup. Environ. Med.

    (1995)
  • M. Ferreira et al.

    Determinants of urinary thioethers, D-glucaric acid and mutagenicity after exposure to PAH assessed by air monitoring and measurements of 1-hydroxypyrene in urine: a cross-sectional study in workers of coke and graphite electrode producing plants

    Int. Arch. Occup. Environ. Health

    (1994)
  • M. Ferreira et al.

    Determinants of benzo(a)pyrene-diolepoxide adducts to haemoglobin in workers exposed to PAH

    Occup. Environ. Med.

    (1994)
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