High-performance liquid chromatographic determination of ibuprofen, its metabolites and enantiomers in biological fluids

doi:10.1016/S0378-4347(00)82827-3

Journal of Chromatography B: Biomedical Sciences and Applications

Volume 491, 1989, Pages 139-149

https://doi.org/10.1016/S0378-4347(00)82827-3 Get rights and content

Abstract

An isocratic high-performance liquid chromatographic method to determine racemic ibuprofen (assay I) and its major metabolites (assay II) in biological fluids (plasma, urine, bile) using a conventional reversed-phase column is described. A third assay using β-cyclodextrin as stationary phase (Cyclobond I) for the separation of the ibuprofen enantiomers is also described. A wavelength of 220 nm was used to monitor the substances. The sensitivity of the method was 0.1 μg/ml for all three assays. The method was demonstrated to be suitable for stereoselective pharmacokinetic studies of ibuprofen in humans and animals.

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High-performance liquid chromatographic determination of ibuprofen, its metabolites and enantiomers in biological fluids

Abstract

J. Pharm. Sci.

J. Chromatogr.

J. Chromatogr.

J. Pharm. Sci.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Chromatogr.

J. Pharm. Sci.

J. Chromatogr.

J. Chromatogr.