Technological advances in high-throughput screening

doi:10.1016/S1367-5931(98)80089-6

Current Opinion in Chemical Biology

Volume 2, Issue 5, 1998, Pages 597-603

https://doi.org/10.1016/S1367-5931(98)80089-6 Get rights and content

Abstract

A variety of assay technologies continue to be developed for high-throughput screening. These include cell-based assays, surrogate systems using microbial cells such as yeast and bacterial two-hybrid and three-hybrid systems, and systems to measure nucleic acid-protein and receptor-ligand interactions. Modifications have been developed for cell-free, homogeneous assay systems, such as time-resolved fluorescence, fluorescence polarization and the scintillation proximity assay. Innovations in engineering and chemistry have led to delivery systems for nanoliter volumes and sensitive biosensors for ultra-high-throughout screening conducted in nanoliter and picoliter volumes. Spectroscopic methods have been extended to read single molecule fluorescence. Technologies are being developed to identify new targets from genomic information in order to design the next generation of screens.

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View full text

Technological advances in high-throughput screening

Abstract

Tetrahedron Letts

J Biol Chem

J Biol Chem

J Biol Chem

Gene

Trends Biotechnol

J Biol Chem

Cell

Drug Discov Today

J Biomol Screening

Anal Biochem

Anal Biochem

J Biomol Screening

Drug Discov Today

Drug Discov Today

Lab Aut News

The current status of high throughput screening and outlook for the future, part 1 (of outstanding interest)

Drug Market Dev

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Spectrum: Drug Discovery and Design. Decision Resources

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Gen Eng News

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Decision Resources

Proteomics

Nat Biotechnol

Proteomics — the new watchword for biotech (of outstanding interest)

Scrip

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Nat Genet

Anatomic localization of alternatively spliced leptin receptors (Ob-R) in mouse brain and other tissues

Proc Natl Acad Sci USA

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Nat Biotechnol

Applications of green fluorescent protein in cell biology and biotechnology (of outstanding interest)

Nat Biotechnol

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Nature