Influence of the Microporous Substratum and Hydrodynamics on Resistances to Drug Transport in Cell Culture Systems: Calculation of Intrinsic Transport Parameters
References and Notes (40)
- et al.
Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability
Gastroenterology
(1989) Epithelial transport of drugs in cell culture. I. A model for studying the passive diffusion of drugs over intestinal absorptive (Caco-2) cells
J. Pharm. Sci
(1990)- et al.
The transport of vitamin B12 through polarized monolayers of Caco-2 cells
Gastroenterology
(1990) - et al.
Transport and permeability properties of human Caco-2 cells: and in vitro model of the intestinal epithelial cell barrier
J. Controlled Release
(1990) - et al.
Permeable support type influences the transport of compounds across Caco-2 cells
Int. J. Pharm.
(1992) - et al.
Heterogeneity in the human intestinal cell line Caco-2 leads to differences in transepithelial transport
Eur. J. Pharm. Sci.
(1995) - et al.
A method for the determination of cellular permeability coefficients and aqueous boundary layer thickness in monolayers of intestinal epithelial (Caco-2) cells grown in permeable filter chambers
Int. J. Pharm.
(1991) - et al.
A new diffusion chamber system for the determination of drug permeability coefficients across the human intestinal epithelium that are independent of the unstirred water layer
Biochim. Biophys. Acta
(1992) - et al.
Quantitative mechanistic studies in simultaneous fluid flow and intestinal absorption using steroids as model solutes
Int. J. Pharm.
(1980) - et al.
H+-coupled (Na+-independent) proline transport in human intestinal (Caco-2) epithelial cell monolayers
FEBS Lett.
(1993)
Determination of intestinal mucosal uptake of short- and medium-chain fatty acids and alcohols
J. Lipid Res.
Sodium-dependent, concentrative nucleoside transport in cultured intestinal epithelial cells
Biochem. Biophys. Res. Commun.
Use of formycin B as a general substrate for measuring facilitated nucleoside transport in mammalian cells
Biochim. Biophys. Acta
Transport of a large neutral amino acid (phenylalanine) in a human intestinal epithelial cell line: Caco-2
Biochim. Biophys. Acta
Metabolism of progesterone, 17-hydroxyprogesterone and deoxycorticosterone by human small intestine in vitro
J. Steroid Biochem.
Functional expression of p-glycoprotein in apical membranes of human intestinal Caco-2 cells
J. Biol. Chem.
Relationship of octanol/buffer and octanol/water partition coefficients to transcellular diffusion across brain microvessel endothelial cell monolayers
Int. J. Pharm.
The use of cultured epithelial and endothelial cells for drug transport and metabolism studies
Pharm. Res.
Caco-2 cell monolayers as a model for drug transport across the intestinal mucosa
Pharm. Res.
Pharmaceutical applications of cell culture: An overview
Cited by (32)
Single-Pass Intestinal Perfusion (SPIP) and prediction of fraction absorbed and permeability in humans: A study with antiretroviral drugs
2016, European Journal of Pharmaceutics and BiopharmaceuticsCitation Excerpt :On the other hand, results in MDCK show a lower permeability, but in that study, MDCK is a canine origin cell line and the expression of transporters is underestimated [52]. Yu and Sinko published a value of 2.16 × 10−5 cm/s using Caco-2 cells [53]. Dezani and colleagues published a value of 53.4 × 10−5 cm/s and this values is greater than the expected [30].
In vitro porcine blood-brain barrier model for permeability studies: PCEL-X software pK<inf>a</inf><sup>FLUX</sup> method for aqueous boundary layer correction and detailed data analysis
2014, European Journal of Pharmaceutical SciencesCitation Excerpt :Also, detailed data analysis in pCEL-X after experiments gives additional information and insights into permeability mechanisms. A similar approach to correct for ABL and refine measured permeability data to derive intrinsic permeability across Caco-2 cells was proposed by Yu and Sinko (1997). Phenylalanine was used as ABL marker.
Determination of lamivudine and zidovudine permeability using a different ex vivo method in Franz cells
2013, Journal of Pharmacological and Toxicological MethodsDetermination of permeation resistance distribution in in vitro cell monolayer permeation experiments
2010, European Journal of Pharmaceutical SciencesAnalysis of unstirred water layer in in vitro permeability experiments
2009, Journal of Pharmaceutical SciencesCitation Excerpt :The latter study suggested that α could be considered as an indicator of stirring efficiency in such a way that a smaller value would indicate better stirring. In addition, values between 0.72 and 0.90 have been reported in a side-by-side chamber using air lift stirring.26 The advantage of this method is that it can be applied to different apparatuses and it yields an estimate for cellular permeability.
Variability in caco-2 and MDCK cell-based intestinal permeability assays
2008, Journal of Pharmaceutical SciencesCitation Excerpt :Markowska et al.86 detected that TEER increased with filter diameter from 6.5 to 12 to 24 mm. In comparing monolayer TEER values, PET filters resulted in lower Papp values than PC filters.7,68,79,82 Pore size (0.4 vs. 3.0 µm) resulted in some difference in testosterone transport,87 while filter diameter changed the Papp for series of drugs but did not change their rank order.86