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Mechanisms for Contractile Effect of Dai-kenchu-to in Isolated Guinea Pig Ileum

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Abstract

The mechanisms by which Dai-kenchu-to (TJ-100), a kampo medicine, enhances gastrointestinal motility was investigated using isolated guinea pig ileum. TJ-100 induced contractions accompanied by autonomous contraction at a concentration of more than 3 × 10−4 g/ml in a dose-related manner. The TJ-100-induced ileal contraction was suppressed by atropine and tetrodotoxin, but not by hexamethonium. This effect was partially suppressed in the presence of high concentrations of ICS 205-930, a serotonin 4 (5-HT4) receptor antagonist. In addition, TJ-100 showed an acetylcholine (ACh) -releasing action in the smooth muscle tissues of ileum. These results suggest that contractile response induced by TJ-100 is partially mediated by ACh released from the cholinergic nerve endings and that 5-HT4 receptors would be involved in the effect of TJ-100.

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Authors and Affiliations

  1. Kampo & Pharmacognosy Laboratory, R & D Division, Tsumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki, 300-1192, Japan

    Kazuko Satoh, Terumasa Hayakawa, Yoshio Kase, Atsushi Ishige, Hiroshi Sasaki, Shigeto Nishikawa, Susumu Kurosawa, Koji Yakabi & Takashi Nakamura

  2. The Third Department of Internal Medicine, Teikyo University, School of Medicine (Ichihara Hospital), 3426-3, Anesaki, Ichihara-shi, Chiba, 299-0111, Japan

    Kazuko Satoh, Terumasa Hayakawa, Yoshio Kase, Atsushi Ishige, Hiroshi Sasaki, Shigeto Nishikawa, Susumu Kurosawa, Koji Yakabi & Takashi Nakamura

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  1. Kazuko Satoh
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  2. Terumasa Hayakawa
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  3. Yoshio Kase
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  4. Atsushi Ishige
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  5. Hiroshi Sasaki
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  6. Shigeto Nishikawa
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  7. Susumu Kurosawa
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  8. Koji Yakabi
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  9. Takashi Nakamura
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Satoh, K., Hayakawa, T., Kase, Y. et al. Mechanisms for Contractile Effect of Dai-kenchu-to in Isolated Guinea Pig Ileum. Dig Dis Sci 46, 250–256 (2001). https://doi.org/10.1023/A:1005636412287

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