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Regulation of the ileal bile acid-binding protein gene: An approach to determine its physiological function(s)

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Abstract

Ileal bile acid-binding protein (I-BABP) is a soluble bile acids (BA) carrier protein which belongs to the fatty acid-binding protein (FABP) family. In the gut, its expression is strictly restricted to the ileum, where it is thought to be involved in the active BA reabsorption. Therefore, I-BABP gene expression levels might be rate limiting for the BA enterohepatic circulation, and hence, might be crucial for cholesterol (CS) homeostasis. Indeed, BA not reclaimed by intestinal absorption constitute the main way to eliminate a CS excess. However, such a function is not yet established. Because generally rate limiting genes are tightly controlled, we have undertaken the study of the I-BABP gene regulation. It was found that both BA and CS, probably via oxysterols, are able to up-regulate the trancription rate of I-BABP gene. The fact that intracellular sterol sensors (FXR, LXR and SREBP1c) are involved in the control of I-BABP gene expression strongly suggest a crucial role for I-BABP in the ileum.

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Authors and Affiliations

  1. Laboratoire de Physiologie de la Nutrition, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation (ENSBANA), FRE 2328 CNRS-CESG/Université de Bourgogne, Dijon, France

    Jean-François Landrier, Jacques Grober & Isabelle Zaghini

  2. Laboratoire de Physiologie de la Nutrition, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation (ENSBANA), FRE 2328 CNRS-CESG/Université de Bourgogne, 1 esplanade Erasme, F21000, Dijon, France

    Philippe Besnard

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  1. Jean-François Landrier
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  2. Jacques Grober
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  3. Isabelle Zaghini
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  4. Philippe Besnard
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Landrier, JF., Grober, J., Zaghini, I. et al. Regulation of the ileal bile acid-binding protein gene: An approach to determine its physiological function(s). Mol Cell Biochem 239, 149–155 (2002). https://doi.org/10.1023/A:1020557502795

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