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Efficient delivery of siRNA for inhibition of gene expression in postnatal mice

Abstract

It has recently been shown that RNA interference can be induced in cultured mammalian cells by delivery of short interfering RNAs (siRNAs). Here we describe a method for efficient in vivo delivery of siRNAs to organs of postnatal mice and demonstrate effective and specific inhibition of transgene expression in a variety of organs.

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Figure 1: RNA interference in organs of mice after injection of siRNA.
Figure 2: Inhibition of EGFP expression in transgenic mice after delivery of siRNA.

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Acknowledgements

We thank J. Hegge, M. Noble, B. Hermanson, M. Finch, K. Vlotho and T. Reppen for technical assistance, and C. Wooddell for critically reading the manuscript.

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Correspondence to David L. Lewis.

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J.A.W. is Chief Scientific Officer and a member of the Board of Directors of Mirus Corporation.

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Lewis, D., Hagstrom, J., Loomis, A. et al. Efficient delivery of siRNA for inhibition of gene expression in postnatal mice. Nat Genet 32, 107–108 (2002). https://doi.org/10.1038/ng944

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