Issue 5, 2009
From the journal:

Analyst

Bioanalysis of recombinant proteins and antibodies by mass spectrometry

Eric Ezan,*a   Mathieu Duboisa  and  François Bechera  

* Corresponding authors

a CEA, IBITECS, Service de Pharmacologie et d'Immunoanalyse, 91191 Gif-sur-Yvette, France
E-mail: eric.ezan@cea.fr
Fax: +33-1-69-08-59-07
Tel: +33-1-69-08-73-50

Abstract

In recent years, biotechnologically-derived drugs have been a major focus of research and development in the pharmaceutical industry. Their pharmacokinetics and pharmacokinetic/pharmacodynamic relationships impact every stage of the development process and require their assessment in the circulation in preclinical species and in humans. To this end, immunoassays are a reference, but standardisation remains an issue owing to the restricted pattern of antibody specificity and interference with endogenous components. As an alternative, we report here analytical strategies involving liquid chromatography coupled to mass spectrometry (LC-MS) for the accurate quantification of therapeutic proteins and antibodies in biological fluids.

Graphical abstract: Bioanalysis of recombinant proteins and antibodies by mass spectrometry

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Article information

DOI
https://doi.org/10.1039/B819706G
Article type
Minireview
First published
24 Mar 2009

Analyst, 2009,134, 825-834

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Bioanalysis of recombinant proteins and antibodies by mass spectrometry

E. Ezan, M. Dubois and F. Becher, Analyst, 2009, 134, 825 DOI: 10.1039/B819706G

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