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Annual Review of Pharmacology and Toxicology

Volume 45, 2005

CYTOCHROME P450: Nature's Most Versatile Biological Catalyst

Abstract

▪ Abstract 

The author describes studies that led to the resolution and reconstitution of the cytochrome P450 enzyme system in microsomal membranes. The review indicates how purification and characterization of the cytochromes led to rigorous evidence for multiple isoforms of the oxygenases with distinct chemical and physical properties and different but somewhat overlapping substrate specificities. Present knowledge of the individual steps in the P450 and reductase reaction cycles is summarized, including evidence for the generation of multiple functional oxidants that may contribute to the exceptional diversity of the reactions catalyzed.

Keyword(s): Autobiography, coupling of reductase and P450 reaction cycles, oxygen activation to give multiple functional oxidants, purification and characterization of multiple P450s, resolution and reconstitution of the microsomal P450 system
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2005-02-10
2024-04-23
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CYTOCHROME P450: Nature's Most Versatile Biological Catalyst
Annual Review of Pharmacology and Toxicology 45, 1 (2005); https://doi.org/10.1146/annurev.pharmtox.45.120403.100030
/content/journals/10.1146/annurev.pharmtox.45.120403.100030
/content/journals/10.1146/annurev.pharmtox.45.120403.100030
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