Abstract
The airway epithelium occupies a critical environmental interface, protecting the host from a wide variety of inhaled insults, including chemical and particulate pollutants and pathogens. The coordinated regulation of ion and water transport, mucous secretion, and cilia beating underlies mucociliary clearance. Physical trapping and removal of harmful substances, in combination with baseline or inducible secretion of antimicrobial factors, antioxidants, and protease inhibitors and recruitment of nonspecific inflammatory cells (neutrophils, monocytes), constitutes airway innate host defense.
Keywords
- Cultured Airway Epithelial Cells (AECCs)
- Human AECCs
- Bronchial Epithelial Growth Medium (BEGM)
- SigmaAldrich
- Collagen-coated Plastic Dishes
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Lechner, J. R, Haugen, A., McLendon, I. A., and Pettis, E. W. (1982) Clonal growth of normal adult human bronchial epithelial cells in a serum-free medium. In Vitro 18, 633–642.
Gruenert, D. C., Finkbeiner, W. E., and Widdicombe, J. H. (1995) Culture and transformation of human airway epithelial cells. Am. J. Physiol. 268, L347–L360.
Terzaghi, M., Nettesheim, P., and Williams, M.L. (1978) Repopulation of denuded tracheal grafts with normal, preneoplastic, and neoplastic epithelial cell populations. Cancer Res. 38, 4546–4553.
Yankaskas, J. R., Knowles, M. R., Gatzy, J. T., and Boucher, R. C. (1985) Persistence of abnormal chloride ion permeability in cystic fibrosis nasal epithelial cells in heterologous culture. Lancet 1, 954–956.
Jorissen, M., Van Der Schueren, B., van den Berghe, H., and Cassiman, J. J. (1989) The preservation and regeneration of cilia on human nasal epithelial cells cultured in vitro. Arch. Otorhinolaryngol. 246, 308–314.
Wu, R., Yankaskas, J., Cheng, E., Knowles, M. R., and Boucher, R. (1985) Growth and differentiation of human nasal epithelial cells in culture. Serum-free, hormone-supplemented medium and proteoglycan synthesis. Am. Rev. Respir. Dis. 132, 311–320.
Benali, R., Tournier, J. M., Chevillard, M., et al. (1993) Tubule formation by human surface respiratory epithelial cells cultured in a three-dimensional collagen lattice. Am. J. Physiol. 264, L183–L192.
Whitcutt, M. J., Adler, K., and Wu, R. (1988) A biphasic chamber system for maintaining polarity of differentiation of cultured respiratory tract epithelial cells. In Vitro Cell. Dev. Biol. 24, 420–428.
Matsui, H., Grubb, B. R., Tarran, R., et al. (1998) Evidence for periciliary liquid layer depletion, not abnormal ion composition, in the pathogenesis of cystic fibrosis airways disease. Cell 95, 1005–1015.
Matsui, H., Randell, S. H., Peretti, S. W., Davis, C. W., and Boucher, R. C. (1998) Coordinated clearance of periciliary liquid and mucus from airway surfaces. J. Clin. Invest. 102, 1125–1131.
Pickles, R. J., McCarty, D., Matsui, H., Hart, P. J., Randell, S. H., and Boucher, R. C. (1998) Limited entry of adenoviral vectors into well differentiated airway epithelium is responsible for inefficient gene transfer. J. Virol. 72, 6014–6023.
Zhang, L., Peeples, M. E., Boucher, R. C., Collins, P. L., and Pickles, R. J. (2002) Respiratory syncytial virus infection of human airway epithelial cells is polarized, specific to ciliated cells, and without obvious cytopathology. J. Virol. 76, 5654–5666.
Bernacki, S. H., Nelson, A. L., Abdullah, L., et al. (1999) Mucin gene expression during differentiation of human airway epithelia in vitro. Muc4 and muc5b are strongly induced. Am. J. Respir. Cell Mol. Biol. 20, 595–604.
Zhang, Y. J., O’Neal, W. K., Randell, S. H., et al. (2002) Identification of dynein heavy chain 7 as an inner arm component of human cilia that is synthesized but not assembled in a case of primary ciliary dyskinesia. J. Biol. Chem. 277, 17,906–17,915.
Yoon, J. H., Koo, J. S., Norford, D., Guzman, K., Gray, T., and Nettesheim, P. (1999) Lysozyme expression during metaplastic squamous differentiation of retinoic acid-deficient human tracheobronchial epithelial cells. Am. J. Respir. Cell Mol. Biol. 20, 573–581.
Gray, T. E., Guzman, K., Davis, C. W., Abdullah, L. H., and Nettesheim, P. (1996) Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells. Am. J. Respir. Cell Mol. Biol. 14, 104–112.
Lechner, J. F. and LaVeck, M. A. (1985) A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tiss. Cult. Meth. 9, 43–48.
Randell, S. H., Walstad, L., Schwab, U. E., Grubb, B. R., and Yankaskas, J. R. (2001) Isolation and culture of airway epithelial cells from chronically infected human lungs. In Vitro Cell Dev. Biol. Anim. 37, 480–489.
Bancroft, J. and Stevens, A. (1996) Theory and Practice of Histological Techniques, Battle Press, Columbus, OH, pp. 69–80.
Sheehan, D. and Hrapchak, B. (1980) Theory and Practice of Histotechnology, Battle Press, Columbus, OH, Vol. 2, 59–66.
Hayat, M. A. (1989) Principles and Techniques of Electron Microscopy 3, 79–92.
Goldstein, J. (1984) Scanning Electron Microscopy and X-ray Micro analysis, pp. 495–540.
Boat, T. E, Welsh, M. J., and Beaudet, A. L. (1989) Cystic fibrosis, in The Metabolic Basis of Inherited Disease (Scriver, C. R., Beaudet, A. L., Sly, W. S., Valle, D., Stansbury, J. B., Wyngaarden, J. B., and Frederickson, D. S., eds.), 2649–2680.
Rommens, J. M., Iannuzzi, M. C., Kerem, B.-T., et al. (1989) Identification of the cystic fibrosis gene: Chromosome walking and jumping. Science 245, 1059–1065.
Riordan, J. R., Rommens, J. M., Kerem, B.-T., et al. (1989) Identification of the cystic fibrosis gene: Cloning and characterization of complementary DNA. Science 245, 1066–1073.
Kerem, B., Rommens, J. M., Buchanan, J. A., et al. (1989) Identification of the cystic fibrosis gene: Genetic analysis. Science 245, 1073–1080.
Anderson, M. P., Rich, D. P., Gregory, R. J., Smith, A. E., and Welsh, M. J. (1991) Generation of cAMP-activated chloride currents by expression of CFTR. Science 251, 679–682.
Kartner, N., Hanrahan, J. W., Jensen, T. J., et al. (1991) Expression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance. Cell 64, 681–691.
Stutts, M. J., Canessa, C. M., Olsen, J. C., et al. (1995) CFTR as a cAMP-dependent regulator of sodium channels. Science 269, 847–850.
Gabriel, S. E., Makhlina, M., Martsen, E., Thomas, E. J., Lethem, M. L, and Boucher, R. C. (2000) Permeabilization via the P2X7 purinoceptor reveals the presence of a Ca2+-activated Cl- conductance in the apical membrane of murine tracheal epithelial cells. J. Biol. Chem. 275, 35,028–35,033.
Tarran, R., Loewen, M. E., Paradiso, A. M., et al. (2002) Regulation of murine airway surface liquid volume by CFTR and Ca2+-activated Cl- conductances. J. Gen. Physiol. 120, 407–418.
Donaldson, S. H., Hirsh, A., Li, D. C., et al. (2002) Regulation of the epithelial sodium channel by serine proteases in human airways. J. Biol. Chem. 277, 8338–8345.
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Fulcher, M.L., Gabriel, S., Burns, K.A., Yankaskas, J.R., Randell, S.H. (2005). Well-Differentiated Human Airway Epithelial Cell Cultures. In: Picot, J. (eds) Human Cell Culture Protocols. Methods in Molecular Medicine™, vol 107. Humana Press. https://doi.org/10.1385/1-59259-861-7:183
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DOI: https://doi.org/10.1385/1-59259-861-7:183
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