Abstract
13-Hydroxyoctadecadienoic acid (13-HODE), hydroxylinoleic acid, is a major lipoxygenase metabolite which is produced by myeloid inflammatory cells and modifies inflammatory cell activity. The biological effects of 13-HODE in non-haemopoietic cells (HUVEC) have been attributed to the incorporation of 13-HODE into 13-HODE containing diacylglycerol and the selective inhibition of protein kinase C. Our studies, using whole promyeloid cells (HL60) and recombinant PKC isoenzymes in an in vitro assay, showed that 13-HODE inhibited PKC-alpha, beta1, and PKC-betall, but did not affect the activity of PKC-delta. These data suggest that the actions of hydroxylinoleic acid on myeloid cells include the selective inhibition of classical PKC isoenzymes.
Copyright 1999 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Membrane / drug effects
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Cell Membrane / enzymology
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Cytosol / drug effects
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Cytosol / enzymology
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Enzyme Activation / drug effects
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HL-60 Cells
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Humans
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Isoenzymes / antagonists & inhibitors
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Isoenzymes / metabolism
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Linoleic Acids / metabolism
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Linoleic Acids / pharmacology*
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Lipoxygenase / metabolism*
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Precipitin Tests
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Protein Kinase C / antagonists & inhibitors*
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Protein Kinase C / metabolism
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Recombinant Proteins / metabolism
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Signal Transduction / drug effects
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Tetradecanoylphorbol Acetate / antagonists & inhibitors
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Tetradecanoylphorbol Acetate / pharmacology
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Time Factors
Substances
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Isoenzymes
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Linoleic Acids
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Recombinant Proteins
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8-hydroxylinoleic acid
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13-hydroxy-9,11-octadecadienoic acid
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Lipoxygenase
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Protein Kinase C
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Tetradecanoylphorbol Acetate