The lipoxygenase product 13-hydroxyoctadecadienoic acid (13-HODE) is a selective inhibitor of classical PKC isoenzymes

Biochem Biophys Res Commun. 1999 Mar 16;256(2):269-72. doi: 10.1006/bbrc.1999.0315.

Abstract

13-Hydroxyoctadecadienoic acid (13-HODE), hydroxylinoleic acid, is a major lipoxygenase metabolite which is produced by myeloid inflammatory cells and modifies inflammatory cell activity. The biological effects of 13-HODE in non-haemopoietic cells (HUVEC) have been attributed to the incorporation of 13-HODE into 13-HODE containing diacylglycerol and the selective inhibition of protein kinase C. Our studies, using whole promyeloid cells (HL60) and recombinant PKC isoenzymes in an in vitro assay, showed that 13-HODE inhibited PKC-alpha, beta1, and PKC-betall, but did not affect the activity of PKC-delta. These data suggest that the actions of hydroxylinoleic acid on myeloid cells include the selective inhibition of classical PKC isoenzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / drug effects
  • Cell Membrane / enzymology
  • Cytosol / drug effects
  • Cytosol / enzymology
  • Enzyme Activation / drug effects
  • HL-60 Cells
  • Humans
  • Isoenzymes / antagonists & inhibitors
  • Isoenzymes / metabolism
  • Linoleic Acids / metabolism
  • Linoleic Acids / pharmacology*
  • Lipoxygenase / metabolism*
  • Precipitin Tests
  • Protein Kinase C / antagonists & inhibitors*
  • Protein Kinase C / metabolism
  • Recombinant Proteins / metabolism
  • Signal Transduction / drug effects
  • Tetradecanoylphorbol Acetate / antagonists & inhibitors
  • Tetradecanoylphorbol Acetate / pharmacology
  • Time Factors

Substances

  • Isoenzymes
  • Linoleic Acids
  • Recombinant Proteins
  • 8-hydroxylinoleic acid
  • 13-hydroxy-9,11-octadecadienoic acid
  • Lipoxygenase
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate