A polymerase chain reaction-based screening method for transgenic Arabidopsis

V De Neef; W Van Caeneghem; M Marichal; M Van Montagu; D Van der Straeten

doi:10.1016/s1050-3862(98)00012-6

A polymerase chain reaction-based screening method for transgenic Arabidopsis

Genet Anal. 1999 Mar;15(1):1-4. doi: 10.1016/s1050-3862(98)00012-6.

Authors

V De Neef¹, W Van Caeneghem, M Marichal, M Van Montagu, D Van der Straeten

Affiliation

¹ Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, Belgium.

PMID: 10084120
DOI: 10.1016/s1050-3862(98)00012-6

Abstract

A simple and rapid PCR-based method for screening transformed Arabidopsis plants has been developed. Based on the quantity of chlorophyll present in a protoplast suspension, the optimal amount of template is calculated and a fragment of the transgene is amplified.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / genetics*
DNA Primers
Plants, Genetically Modified / genetics
Polymerase Chain Reaction / methods*
Reproducibility of Results

Substances

DNA Primers