Abstract
We previously developed a two-plasmid in vitro ligation method that did not require a recombination step to produce new recombinant E1- or E1/E3-deleted adenoviral vectors. In this study, we have modified the system to improve the simplicity of vector construction and, in addition, to allow for production of an E1/E4-deleted vector.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae / genetics*
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Adenovirus E1 Proteins / genetics*
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Adenovirus E3 Proteins / genetics
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Adenovirus E4 Proteins / genetics*
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Gene Deletion
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Genetic Vectors / genetics*
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Plasmids / genetics
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Recombinant Proteins / genetics*
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beta-Galactosidase / metabolism
Substances
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Adenovirus E1 Proteins
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Adenovirus E3 Proteins
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Adenovirus E4 Proteins
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Recombinant Proteins
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beta-Galactosidase