Abstract
Treatment of Caco-2 cells with the antioxidants quercetin or t-butylhydroquinone led to induced protein levels of UDP-glucuronosyltransferase UGT1A6 (ca. 3-fold over controls) and of the apical conjugate export pump multidrug resistance protein 2 (MRP2; 1.9-fold over controls). In contrast to UGT1A6, MRP2 (symbol ABCC2) was not inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Immunocytochemistry demonstrated that MRP2 was only expressed at the brush border domain of Caco-2 cell monolayers. The results indicate that UGT1A6 and MRP2 are coordinately induced by antioxidants, facilitating chemoprotection against phenolic toxins and excretion of conjugates into the intestinal lumen.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B / biosynthesis*
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ATP-Binding Cassette Transporters / biosynthesis*
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Antioxidants / pharmacology
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Caco-2 Cells
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Glucuronosyltransferase / biosynthesis*
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Humans
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Immunoblotting
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Membrane Transport Proteins*
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Multidrug Resistance-Associated Protein 2
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Multidrug Resistance-Associated Proteins
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Polychlorinated Dibenzodioxins / pharmacology
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Quercetin / pharmacology
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Receptors, Aryl Hydrocarbon / agonists
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Teratogens / pharmacology
Substances
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ABCC2 protein, human
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ATP Binding Cassette Transporter, Subfamily B
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ATP-Binding Cassette Transporters
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Antioxidants
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Membrane Transport Proteins
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Multidrug Resistance-Associated Protein 2
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Multidrug Resistance-Associated Proteins
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Polychlorinated Dibenzodioxins
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Receptors, Aryl Hydrocarbon
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Teratogens
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Quercetin
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UDP-glucuronosyltransferase, UGT1A6
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Glucuronosyltransferase
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multidrug resistance-associated protein 1