Molecular identification and characterization of novel members of the human organic anion transporter (OATP) family

Biochem Biophys Res Commun. 2000 Jun 24;273(1):251-60. doi: 10.1006/bbrc.2000.2922.

Abstract

We identified three novel transporters structurally belonging to the organic anion transporting polypeptide (OATP) family in humans. Since previously known rat oatp1 to 3 do not necessarily correspond to the human OATPs in terms of either tissue distribution or function, here we designate the newly identified human OATPs as OATP-B, -D and -E, and we rename the previously known human OATP as OATP-A. OATP-C proved to be identical with the recently reported LST1/OATP-2. Expression profiles of the five OATPs and the prostaglandin transporter PGT (a member of OATP family) in human tissues showed that OATP-C is exclusively localized in liver, OATP-A and PGT are expressed in restricted ranges of tissues, and OATP-B, -D and -E show broad expression profiles. OATP-B, -C, -D and -E exhibited transport activity for [(3)H]estrone-3-sulfate as a common substrate. OATP-C has a high transport activity with broad substrate specificity.

MeSH terms

  • Amino Acid Sequence
  • Anion Transport Proteins
  • Antiporters / chemistry
  • Antiporters / genetics
  • Biological Transport
  • Carrier Proteins / chemistry
  • Carrier Proteins / classification
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • Cell Line
  • Cloning, Molecular
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • Dinoprostone / metabolism
  • Estradiol / analogs & derivatives
  • Estradiol / metabolism
  • Estrone / analogs & derivatives
  • Estrone / metabolism
  • Gene Expression Profiling
  • Humans
  • Molecular Sequence Data
  • Multigene Family / genetics*
  • Organ Specificity
  • Organic Anion Transporters
  • Penicillin G / metabolism
  • Phylogeny
  • Physical Chromosome Mapping
  • Polymorphism, Single Nucleotide / genetics
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Substrate Specificity
  • Transfection

Substances

  • Anion Transport Proteins
  • Antiporters
  • Carrier Proteins
  • DNA-Binding Proteins
  • Organic Anion Transporters
  • RNA, Messenger
  • SLCO2A1 protein, human
  • Slco2a1 protein, rat
  • estradiol-17 beta-glucuronide
  • Estrone
  • Estradiol
  • Dinoprostone
  • Penicillin G
  • estrone sulfate

Associated data

  • GENBANK/AB026256
  • GENBANK/AB026257
  • GENBANK/AB031050
  • GENBANK/AB031051