Use of a microplate scintillation counter as a radioactivity detector for miniaturized separation techniques in drug metabolism

Anal Chem. 2000 Aug 15;72(16):3956-9. doi: 10.1021/ac000432s.

Abstract

In miniaturized separation techniques, such as capillary electrophoresis (CE) or capillary liquid chromatography (LC), conventional on-line radioactivity detection of labeled compounds is restricted, because of insufficient sensitivity. It will be shown that a microplate scintillation counter for 96-well plates (TopCount) can be used as a sensitive and easy-to-handle radioactivity detector for capillary LC and CE. The attractive combination of capillary LC, eluent fractionation, and subsequent off-line counting is described. The new method is applied for rapid and sensitive separation and detection of 3H-labeled parent drug and its metabolites at levels between 25 and 700 cpm in rat urine. The advantages of capillary LC coupled to the TopCount, and combined with LC-MS data, can be of benefit in many analytical areas, including the characterization of metabolites at low concentration within complex biological fluids. With the same setup, the fractionation with subsequent off-line counting is equally applicable to CE. This is demonstrated with electrophoretically separated 14C-labeled impurities, nicely resolved from a negatively charged main compound, at low levels.

MeSH terms

  • Animals
  • Miniaturization
  • Pharmaceutical Preparations / metabolism*
  • Radiochemistry
  • Rats
  • Scintillation Counting / methods*
  • Urine / chemistry

Substances

  • Pharmaceutical Preparations