Abstract
Two reductases, P450 oxidoreductase and P450Bm-3 reductase, were purified on a 2',5'-adenosine diphosphate solid support. Although the efficiency of these columns is well established, the cost of the resin and the eluting material 2'-adenosine can be prohibitive. Herein we show that the less costly 2',3'-adenosine monophosphate is an excellent eluting material.
Copyright 2001 Academic Press.
MeSH terms
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Adenosine Diphosphate / metabolism
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Adenosine Monophosphate / metabolism
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Bacterial Proteins*
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Chromatography, Affinity*
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Chromatography, Agarose*
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Cytochrome P-450 Enzyme System / isolation & purification*
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Cytochrome P-450 Enzyme System / metabolism
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Mixed Function Oxygenases / isolation & purification*
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Mixed Function Oxygenases / metabolism
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NADPH-Ferrihemoprotein Reductase / isolation & purification*
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NADPH-Ferrihemoprotein Reductase / metabolism
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Protein Binding
Substances
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Bacterial Proteins
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adenosine 2',5'-diphosphate
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Adenosine Monophosphate
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Adenosine Diphosphate
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Cytochrome P-450 Enzyme System
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Mixed Function Oxygenases
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NADPH-Ferrihemoprotein Reductase
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flavocytochrome P450 BM3 monoxygenases