Regulation of the expression of two female-predominant CYP3A mRNAs (CYP3A41 and CYP3A44) in mouse liver by sex and growth hormones

Tsutomu Sakuma; Yusuke Endo; Misae Mashino; Masami Kuroiwa; Ayako Ohara; Kanokwan Jarukamjorn; Nobuo Nemoto

doi:10.1016/s0003-9861(02)00329-6

Regulation of the expression of two female-predominant CYP3A mRNAs (CYP3A41 and CYP3A44) in mouse liver by sex and growth hormones

Arch Biochem Biophys. 2002 Aug 15;404(2):234-42. doi: 10.1016/s0003-9861(02)00329-6.

Authors

Tsutomu Sakuma¹, Yusuke Endo, Misae Mashino, Masami Kuroiwa, Ayako Ohara, Kanokwan Jarukamjorn, Nobuo Nemoto

Affiliation

¹ Department of Toxicology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan. tsakuma@ms.toyama-mpu.ac.jp

PMID: 12147261
DOI: 10.1016/s0003-9861(02)00329-6

Abstract

A second female-predominant murine CYP3A, CYP3A44, was isolated from liver and its mRNA expression was compared with that of the previously described CYP3A41. The expression of CYP3A44 was relatively constant after birth in females, whereas it gradually declined in males after 5 weeks of age. The expression of CYP3A41 increased with age in females after 3 weeks of age, whereas it gradually declined in males after 5 weeks of age. Hypophysectomy and growth hormone replacement indicated that expression of both CYP3A mRNAs in females was dependent on the feminine plasma growth hormone profile. Estradiol induced the expression of both mRNAs and the effect was dependent on the presence of the pituitary gland. These observations suggest that endocrine control of expression might be similar, but not identical, for two female-predominant CYP3A mRNAs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Aryl Hydrocarbon Hydroxylases*
Base Sequence
Castration
Cytochrome P-450 CYP3A
Cytochrome P-450 Enzyme System / genetics*
Cytochrome P-450 Enzyme System / metabolism
DNA, Complementary / genetics
DNA, Complementary / isolation & purification
Estradiol / pharmacology
Female
Gene Expression / drug effects
Gene Expression Regulation, Developmental / physiology*
Growth Hormone / pharmacology
Hypophysectomy
Hypothalamo-Hypophyseal System / physiology
Liver / drug effects
Liver / enzymology*
Male
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Organ Specificity
Ovariectomy
Oxidoreductases / genetics*
Oxidoreductases / metabolism
Oxidoreductases, N-Demethylating / genetics*
Oxidoreductases, N-Demethylating / metabolism
RNA, Messenger / metabolism*
Sex Factors
Testosterone / pharmacology

Substances

Cyp3a44 protein, mouse
DNA, Complementary
RNA, Messenger
Testosterone
Estradiol
Growth Hormone
Cytochrome P-450 Enzyme System
Cyp3a41a protein, mouse
Oxidoreductases
Aryl Hydrocarbon Hydroxylases
Cytochrome P-450 CYP3A
Oxidoreductases, N-Demethylating

Associated data

GENBANK/AB039380