Abstract
The nuclear receptor CAR, a phenobarbital (PB)-responsive transcription factor, translocates into the nucleus of hepatocytes after PB induction. In non-induced mice, CAR forms a physical complex with heat shock protein 90 (HSP90) in the cytoplasm. In response to PB induction, protein phosphatase 2A is recruited to the CAR:HSP90 complex. This recruitment may lead CAR to translocate into the nucleus, consistent with the inhibitions of nuclear CAR accumulation in PB-induced mouse primary hepatocytes by okadaic acid as well as by geldanamycin.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Active Transport, Cell Nucleus / physiology
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Animals
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Constitutive Androstane Receptor
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Cytosol / chemistry
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HSP90 Heat-Shock Proteins / metabolism*
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Hepatocytes / chemistry
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Hepatocytes / ultrastructure
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Liver / chemistry*
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Liver / cytology
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Mice
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Mice, Inbred Strains
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Phenobarbital / pharmacology*
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Phosphoprotein Phosphatases / metabolism*
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Protein Phosphatase 2
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Protein Transport / physiology
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Receptors, Cytoplasmic and Nuclear / metabolism*
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Transcription Factors / metabolism*
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Transcriptional Activation
Substances
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Constitutive Androstane Receptor
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HSP90 Heat-Shock Proteins
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Receptors, Cytoplasmic and Nuclear
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Transcription Factors
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Phosphoprotein Phosphatases
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Protein Phosphatase 2
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Phenobarbital