Abstract
A full-length cDNA complementary to mouse liver mRNA coding for one of the cytochromes P-450 (P-450) in the P-450IIIA family, namely P-450IIIM1, was isolated and completely sequenced. The sequence of this cDNA clone, pMDex13, revealed that it encoded a polypeptide of 504 deduced amino acid residues (Mr = 57,853). The deduced amino acid sequence showed 87.3 and 84.9% identity with rat P-450IIIA1 and P-450IIIA2, respectively. The NH2-terminal 24 amino acid sequences of P-450IIIAM1 were completely identical with purified mouse P-450UT protein. RNA blot analysis showed that mRNA content of hepatic P-450IIIAM1 was remarkably increased by treatment of mice with dexamethasone.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cloning, Molecular
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Cytochrome P-450 CYP3A
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Cytochrome P-450 Enzyme System / chemistry
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Cytochrome P-450 Enzyme System / genetics*
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Dexamethasone / pharmacology*
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Gene Expression Regulation / drug effects*
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Liver
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Membrane Proteins
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Mice
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Molecular Sequence Data
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Sequence Homology, Nucleic Acid
Substances
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Membrane Proteins
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Dexamethasone
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Cytochrome P-450 Enzyme System
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Cyp3a11 protein, mouse
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Cytochrome P-450 CYP3A
Associated data
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GENBANK/M55186
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GENBANK/M55187
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GENBANK/M55188
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GENBANK/M91426
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GENBANK/M91427
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GENBANK/M91428
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GENBANK/M91429
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GENBANK/M91430
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GENBANK/X60452
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GENBANK/X61944