Abstract
When N-alkylprotoporphyrins are prepared synthetically or biologically, a mixture of four regioisomers is obtained. For our studies, separation of the potent ferrochelatase-inhibitory ring A (NA) and ring B (NB) regioisomers from the ring C (NC) and ring D (ND) regioisomers of low potency is required. Previously this separation required two successive high-performance liquid chromatography procedures. We now report that the separation of the zinc complexes of the NA and NB regioisomers from the NC and ND regioisomers can be achieved by a rapid and inexpensive thin-layer chromatography procedure.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chromatography, Thin Layer / methods*
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Dicarbethoxydihydrocollidine / administration & dosage
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Dicarbethoxydihydrocollidine / analogs & derivatives
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Ferrochelatase / antagonists & inhibitors*
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Isomerism
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Liver / chemistry
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Liver / drug effects
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Male
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Protoporphyrins / isolation & purification*
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Protoporphyrins / metabolism
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Protoporphyrins / pharmacology
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Rats
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Rats, Sprague-Dawley
Substances
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Protoporphyrins
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3,5-dicarbethoxy-2,6-dimethyl-4-ethyl-1,4-dihydropyridine
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Dicarbethoxydihydrocollidine
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N-ethylprotoporphyrin IX
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Ferrochelatase