Thin-layer chromatographic separation of the ferrochelatase-inhibitory ring A and ring B regioisomers of N-ethylprotoporphyrin from a mixture of the four regioisomers

J Pharmacol Toxicol Methods. 1992 Sep;28(2):113-7. doi: 10.1016/1056-8719(92)90056-7.

Abstract

When N-alkylprotoporphyrins are prepared synthetically or biologically, a mixture of four regioisomers is obtained. For our studies, separation of the potent ferrochelatase-inhibitory ring A (NA) and ring B (NB) regioisomers from the ring C (NC) and ring D (ND) regioisomers of low potency is required. Previously this separation required two successive high-performance liquid chromatography procedures. We now report that the separation of the zinc complexes of the NA and NB regioisomers from the NC and ND regioisomers can be achieved by a rapid and inexpensive thin-layer chromatography procedure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Thin Layer / methods*
  • Dicarbethoxydihydrocollidine / administration & dosage
  • Dicarbethoxydihydrocollidine / analogs & derivatives
  • Ferrochelatase / antagonists & inhibitors*
  • Isomerism
  • Liver / chemistry
  • Liver / drug effects
  • Male
  • Protoporphyrins / isolation & purification*
  • Protoporphyrins / metabolism
  • Protoporphyrins / pharmacology
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Protoporphyrins
  • 3,5-dicarbethoxy-2,6-dimethyl-4-ethyl-1,4-dihydropyridine
  • Dicarbethoxydihydrocollidine
  • N-ethylprotoporphyrin IX
  • Ferrochelatase