Abstract
Multidrug resistance transporter MRP1 could be effectively inhibited by some flavonoids. The influence of the two pairs of isoflavones: formononetin/daidzein and biochanin A/genistein on the efflux of fluorescent substrate of MRP1-like protein from erythrocytes and biophysical properties of lipid membranes has been compared. Compounds in each pair differ by the substituent in position 4' of B ring of isoflavone molecule. In the process of O-demethylation, CH(3) group (present in formonetin and biochanin A) is replaced by hydrogen (daidzein, genistein). Inhibition of MRP1-like protein transport activity by methylated and demethylated isoflavones was very similar. Their influence on lipid thermotropic properties and fluidity of lipid bilayer was not also significantly different.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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1,2-Dipalmitoylphosphatidylcholine / metabolism*
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Adult
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Anti-Inflammatory Agents, Non-Steroidal / metabolism
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Anticarcinogenic Agents / chemistry
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Anticarcinogenic Agents / metabolism*
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Biophysical Phenomena
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Biophysics*
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Dihydropyridines / chemistry
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Erythrocytes / metabolism*
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Female
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Genes, MDR
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Genistein / chemistry
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Genistein / metabolism
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Humans
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Indomethacin / metabolism
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Isoflavones / chemistry
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Isoflavones / metabolism*
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Male
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Methylation
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Middle Aged
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Molecular Structure
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Multidrug Resistance-Associated Proteins / metabolism*
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Octanols / chemistry
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Plant Preparations / chemistry
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Plant Preparations / metabolism
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Reference Standards
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Temperature
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Thermodynamics
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Water / chemistry
Substances
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2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylic acid monomethyl ester
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Anti-Inflammatory Agents, Non-Steroidal
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Anticarcinogenic Agents
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Dihydropyridines
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Isoflavones
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Multidrug Resistance-Associated Proteins
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Octanols
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Plant Preparations
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Water
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1,2-Dipalmitoylphosphatidylcholine
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formononetin
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daidzein
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Genistein
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biochanin A
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Indomethacin
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multidrug resistance-associated protein 1