Abstract
The protective properties of irisolidone (a metabolite of kakkalide by intestinal bacteria) against hydrogen peroxide (H(2)O(2)) induced cell damage were investigated. Irisolidone was found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and the intracellular reactive oxygen species (ROS), thereby preventing lipid peroxidation and DNA damage. Irisolidone inhibited apoptosis in Chinese hamster lung fibroblast (V79-4) cells induced by H(2)O(2) via radical scavenging activity. This was achieved by the activation of the extracellular signal regulated kinase (ERK) and DNA binding activity of activator protein-1 (AP-1) (a downstream transcription factor of ERK) by irisolidone.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / metabolism*
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Apoptosis / drug effects*
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Cell Line
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Cricetinae
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Cytoprotection / drug effects
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DNA Damage / genetics
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Enzyme Activation / drug effects
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Flavonoids / chemistry
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Flavonoids / pharmacology*
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Free Radicals / metabolism
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Glycosides / chemistry
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Glycosides / metabolism*
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Humans
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Hydrogen Peroxide / pharmacology*
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Intestinal Mucosa / metabolism
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Intestines / microbiology
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Isoflavones / chemistry
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Isoflavones / metabolism*
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Lipid Peroxidation / drug effects
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Molecular Structure
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Transcription Factor AP-1 / metabolism
Substances
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Antioxidants
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Flavonoids
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Free Radicals
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Glycosides
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Isoflavones
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Transcription Factor AP-1
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irisolidone
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kakkalide
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Hydrogen Peroxide
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Extracellular Signal-Regulated MAP Kinases