Abstract
In many organisms, glycogen gives rise to 1,5-anhydro-D-fructose (AF), which is reduced to 1,5-anhydro-D-glucitol (AG). AF reductase, which catalyzes the latter reaction, was purified from pig liver, but mouse ortholog has not yet been reported. In the database, aldo-keto reductase family 1, member E1 (AKR1E1) showed highest homology to pig enzyme. We confirmed that cloned AKR1E1 is mouse ortholog based on enzymatic properties of purified recombinant protein.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alcohol Oxidoreductases / chemistry*
-
Alcohol Oxidoreductases / genetics
-
Alcohol Oxidoreductases / isolation & purification
-
Aldehyde Reductase
-
Aldo-Keto Reductases
-
Amino Acid Sequence
-
Animals
-
Cloning, Molecular
-
Mice
-
Molecular Sequence Data
-
Sequence Alignment
-
Sequence Homology, Amino Acid
-
Sugar Alcohol Dehydrogenases / chemistry*
-
Sugar Alcohol Dehydrogenases / isolation & purification
-
Sus scrofa
Substances
-
Alcohol Oxidoreductases
-
Sugar Alcohol Dehydrogenases
-
1,5-anhydro-fructose reductase
-
Aldo-Keto Reductases
-
Aldehyde Reductase
-
AKR7A5 protein, mouse