Abstract
A sensitive nonradioisotopic method is reported for measuring microsomal lauric acid omega-hydroxylation activity. The assay is based upon separation and detection of 12-hydroxylauric acid formed by means of high-performance liquid chromatography following fluorescence labeling of the carboxyl group with 3-bromomethyl-7-methoxy-1,4-benzoxazin-2-one (BrMB). The use of 10-hydroxycapric acid as an internal standard affords the accurate and reproducible assay. The differential effect of dehydroepiandrosterone, a peroxisome proliferator, on the omega-hydroxylation activity in the liver and kidney of rats is also reported.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzoxazines
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Chromatography, High Pressure Liquid / methods
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Dehydroepiandrosterone / pharmacology
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Fluorescent Dyes
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Hydroxy Acids / analysis*
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Hydroxy Acids / metabolism
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Hydroxylation
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Kidney Cortex / drug effects
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Kidney Cortex / enzymology*
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Lauric Acids / analysis*
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Lauric Acids / metabolism
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Male
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Microsomes / drug effects
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Microsomes / enzymology*
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Microsomes, Liver / drug effects
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Microsomes, Liver / enzymology*
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Oxazines*
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Rats
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Rats, Inbred Strains
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Reference Values
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Spectrometry, Fluorescence
Substances
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Benzoxazines
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Fluorescent Dyes
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Hydroxy Acids
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Lauric Acids
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Oxazines
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lauric acid
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3-bromomethy-7-methoxy-1,4-benzoxazin-2-one
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Dehydroepiandrosterone