Microbial models of mammalian metabolism: production of 3'-hydroxywarfarin, a new metabolite of warfarin using Cunninghamella elegans

Y W Wong; P J Davis

doi:10.1002/jps.2600800402

Microbial models of mammalian metabolism: production of 3'-hydroxywarfarin, a new metabolite of warfarin using Cunninghamella elegans

J Pharm Sci. 1991 Apr;80(4):305-8. doi: 10.1002/jps.2600800402.

Authors

Y W Wong¹, P J Davis

Affiliation

¹ Division of Medicinal Chemistry, College of Pharmacy, University of Texas, Austin 78712.

PMID: 1865328
DOI: 10.1002/jps.2600800402

Abstract

Warfarin, an anticoagulant and "metabolic probe" for cytochrome P-450 isozyme multiplicity, was metabolized by the fungus Cunninghamella elegans (ATCC 36112) to yield the previously unreported metabolite 3'-hydroxywarfarin. This metabolite was isolated from cell suspension cultures and characterized by analytical (HPLC) and spectral (EI-MS, PMR) comparisons with synthetic 3'-hydroxywarfarin.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Magnetic Resonance Spectroscopy / methods
Mass Spectrometry / methods
Molecular Structure
Mucorales / metabolism*
Warfarin / analogs & derivatives*
Warfarin / metabolism*

Substances

3'-hydroxywarfarin
Warfarin

Grants and funding

R01-ES04200/ES/NIEHS NIH HHS/United States