Abstract
Enzyme kinetic parameters for midazolam were time-dependent in human liver microsomes, under initial velocity conditions. V(max) and K(m) decreased up to 3.7 and 3.1-fold, respectively, for 10 min compared to 1 min incubations. Mathematical models describing the relationship between inactivation and the time-dependency of enzyme kinetic parameter estimates were derived and discussed.
MeSH terms
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Algorithms
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Biotransformation
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Chromatography, High Pressure Liquid
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Computer Simulation
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Data Interpretation, Statistical
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Enzymes / metabolism*
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Humans
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Hypnotics and Sedatives / pharmacokinetics*
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In Vitro Techniques
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Kinetics
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Midazolam / pharmacokinetics*
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Models, Statistical
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Spectrometry, Mass, Electrospray Ionization
Substances
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Enzymes
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Hypnotics and Sedatives
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Midazolam