Abstract
A 1.96 kbp cDNA encoding a male Beagle dog liver cytochrome P-450 of 503 amino acid residues (Mr 57,636) has been isolated and sequenced. The deduced amino acid sequence is 79.8%, 69.3% and 74.1% identical to the P450IIIA forms human NF25, rat PCN1 and rabbit LM3c, respectively. The amino terminal sequence is identical to the first 28 residues of the dog P450IIIA form PBD-1. Southern blot analysis yields restriction patterns consistent with IIIA gene subfamily multiplicity.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cytochrome P-450 Enzyme System / genetics*
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DNA / genetics*
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DNA / isolation & purification
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Dogs
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Humans
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Liver / enzymology*
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Male
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Molecular Sequence Data
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Molecular Weight
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Multigene Family*
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Rabbits
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Sequence Homology, Nucleic Acid
Substances
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DNA
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Cytochrome P-450 Enzyme System
Associated data
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GENBANK/M75971
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GENBANK/M75972
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GENBANK/M75973
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GENBANK/M75974
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GENBANK/M75975
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GENBANK/S57579
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GENBANK/S59357
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GENBANK/X54747
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GENBANK/X54748
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GENBANK/X54915