Abstract
Sandwich-cultured hepatocytes (SCH) are a powerful in vitro tool that can be utilized to study hepatobiliary drug transport, species differences in drug transport, transport protein regulation, drug-drug interactions, and hepatotoxicity. This review provides an up-to-date summary of the SCH model, including a brief history of, and introduction to, the use of SCH, as well as methodology to evaluate hepatobiliary drug disposition. A summary of the literature that has utilized this model to examine the interplay between drug-metabolizing enzymes and transport proteins, drug-drug interactions at the transport level, and hepatotoxicity as a result of altered hepatic transport also is provided.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Animals
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Animals, Genetically Modified
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Bile Acids and Salts / metabolism*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Culture Techniques* / methods
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Chemical and Drug Induced Liver Injury / metabolism*
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Chemical and Drug Induced Liver Injury / pathology*
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Drug Interactions
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Hepatocytes / metabolism
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Hepatocytes / pathology
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Hepatocytes / physiology*
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Humans
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Liver / metabolism
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism*
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Pharmaceutical Preparations / metabolism
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Predictive Value of Tests
Substances
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Bile Acids and Salts
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Carrier Proteins
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Membrane Glycoproteins
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Pharmaceutical Preparations
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bile acid binding proteins