Abstract
A human lymphoblastoid cell line stably expressing a human cytochrome P450IA2 cDNA was developed. This recombinant cell line displayed P450IA2 protein and estradiol 2-hydroxylase activity, neither of which was detected in the parental cell line. The recombinant cell line was also approximately 1000-fold more sensitive to the cytotoxicity and mutagenicity of the carcinogenic mycotoxin aflatoxin B1 than was the parent cell line. The increase in mutagenicity was supported by a corresponding increase in the level of aflatoxin B1 binding to DNA in cells expressing P450IA2 relative to control cells.
MeSH terms
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Aflatoxin B1
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Aflatoxins / metabolism*
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Aflatoxins / toxicity
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B-Lymphocytes
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Biotransformation
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Blotting, Western
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Cell Division / drug effects
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Cell Line
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Cytochrome P-450 CYP1A1*
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Cytochrome P-450 Enzyme System / biosynthesis
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Cytochrome P-450 Enzyme System / genetics
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Cytochrome P-450 Enzyme System / metabolism*
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DNA / biosynthesis
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DNA / genetics
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DNA / metabolism*
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Gene Expression*
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Humans
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Mutagens
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Steroid Hydroxylases / metabolism
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Transfection
Substances
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Aflatoxins
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Mutagens
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DNA
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Cytochrome P-450 Enzyme System
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Aflatoxin B1
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Steroid Hydroxylases
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Cytochrome P-450 CYP1A1
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estrogen 2-hydroxylase