Abstract
We present here an initial characterization of ATP binding cassette (ABC) transporter function and regulation at the blood-spinal cord barrier. We isolated capillaries from rat spinal cords and studied transport function using a confocal microscopy-based assay and protein expression using western blots. These capillaries exhibited transport function and protein expression of P-glycoprotein (Abcb1), multidrug resistance protein 2 (Mrp2, Abcc2), and breast cancer-related protein (Bcrp, Abcg2). Exposing isolated capillaries to dioxin (activates aryl hydrocarbon receptor) increased transport mediated by all three transporters. Brain and spinal cord capillaries from dioxin-dosed rats exhibited increased P-glycoprotein-mediated transport and increased protein expression for all three ABC transporters. These findings indicate similar ABC transporter expression, function, and regulation at the blood-spinal cord and blood-brain barriers.
Publication types
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Research Support, N.I.H., Intramural
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B* / genetics
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ATP Binding Cassette Transporter, Subfamily B* / metabolism
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ATP Binding Cassette Transporter, Subfamily G, Member 2
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ATP-Binding Cassette Transporters* / genetics
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ATP-Binding Cassette Transporters* / metabolism
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Animals
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Biological Transport
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Blood-Brain Barrier / metabolism*
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Blotting, Western
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Capillaries / metabolism
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Cell Membrane / metabolism
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Electrophoretic Mobility Shift Assay
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Ligands
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Male
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Mice
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Mice, Knockout
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Microscopy, Confocal
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Polychlorinated Dibenzodioxins / pharmacokinetics
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Rats
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Rats, Sprague-Dawley
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Spinal Cord / blood supply*
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Spinal Cord / metabolism*
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Xenobiotics / pharmacokinetics
Substances
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ATP Binding Cassette Transporter, Subfamily B
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ATP Binding Cassette Transporter, Subfamily G, Member 2
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ATP-Binding Cassette Transporters
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Abcc2 protein, rat
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Abcg2 protein, rat
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Ligands
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Polychlorinated Dibenzodioxins
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Xenobiotics
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multidrug resistance protein 3