No correlation of hsa-miR-148a with expression of PXR or CYP3A4 in human livers from Chinese Han population

Zhiyun Wei; Mingjie Chen; Yiting Zhang; Xiaofei Wang; Songshan Jiang; Yang Wang; Xi Wu; Shengying Qin; Lin He; Lirong Zhang; Qinghe Xing

doi:10.1371/journal.pone.0059141

No correlation of hsa-miR-148a with expression of PXR or CYP3A4 in human livers from Chinese Han population

PLoS One. 2013;8(3):e59141. doi: 10.1371/journal.pone.0059141. Epub 2013 Mar 20.

Authors

Zhiyun Wei¹, Mingjie Chen, Yiting Zhang, Xiaofei Wang, Songshan Jiang, Yang Wang, Xi Wu, Shengying Qin, Lin He, Lirong Zhang, Qinghe Xing

Affiliation

¹ Children's Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai, People's Republic of China.

Abstract

There is a huge variability of hepatic CYP3A4 level in human populations, which was believed to contribute to different responses to drugs among individuals. Transcription of CYP3A4 was regulated by transcription factors such as pregnane X receptor (PXR). MiRNA hsa-miR-148a was previously reported to influence PXR expression in HepG2 cells and in Japanese populations. In this study, we conducted a similar correlation study in Chinese Han population (N = 24). No significant correlation of hsa-miR-148a was found with PXR expression or CYP3A4 expression. Our results suggest that hsa-miR-148a does not play a major role in the regulation of PXR or CYP3A4 expression in human livers from Chinese Han population.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Asian People / genetics
China
Cytochrome P-450 CYP3A / genetics*
Cytochrome P-450 CYP3A / metabolism
Female
Gene Expression Regulation*
Humans
Liver / metabolism*
Liver Cirrhosis / genetics
Liver Cirrhosis / metabolism
Male
MicroRNAs / genetics*
Middle Aged
Pregnane X Receptor
RNA, Messenger / genetics
RNA, Messenger / metabolism
Receptors, Steroid / genetics*
Receptors, Steroid / metabolism

Substances

MIRN148 microRNA, human
MicroRNAs
Pregnane X Receptor
RNA, Messenger
Receptors, Steroid
Cytochrome P-450 CYP3A
CYP3A4 protein, human

Grants and funding

This work was supported by the National Natural Science Foundation of China [grant 30971582; 90919049; 81173127], and the 973 Program [2010CB529600]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.