Abstract
Sulforhodamine 101 (SR101) is widely used for astrocyte identification, though the labeling mechanism remains unknown and the efficacy of labeling in different brain regions is heterogeneous. By combining region-specific isolation of astrocytes followed by transcriptome analysis, two-photon excitation microscopy, and mouse genetics, we identified the thyroid hormone transporter OATP1C1 as the SR101-uptake transporter in hippocampus and cortex.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Astrocytes / metabolism*
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Cerebral Cortex / cytology
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Coloring Agents*
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Computational Biology
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Electrolytes / cerebrospinal fluid
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Female
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Hippocampus / cytology*
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In Vitro Techniques
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Male
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Mice
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Mice, Transgenic
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Optogenetics
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Organic Cation Transport Proteins / genetics
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Organic Cation Transport Proteins / metabolism*
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Rhodamines*
Substances
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Coloring Agents
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Electrolytes
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Oatp2 protein, mouse
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Organic Cation Transport Proteins
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Rhodamines
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Green Fluorescent Proteins
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sulforhodamine 101