Abstract
7-Dehydrocholesterol sulphate has been identified in human and rat skin. The compound was isolated by anion exchange chromatography and following hydrolysis it was characterized by high-performance liquid chromatography and gas chromatography-mass spectrometry. Experiments with rats showed that 7-dehydrocholesterol sulphate can serve as a precursor of cholecalciferol sulphate and 25-hydroxy-cholecalciferol 3-sulphate, the latter compound being present in significant amounts in human blood. The sulphated sterols identified represent a previously unknown secosteroid system in mammals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcifediol / analogs & derivatives
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Calcifediol / metabolism
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Cholecalciferol / metabolism*
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Cholestadienols / analysis*
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Chromatography, High Pressure Liquid
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Chromatography, Ion Exchange
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Dehydrocholesterols / analysis*
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Dehydrocholesterols / metabolism
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Female
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Gas Chromatography-Mass Spectrometry
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Humans
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Rats
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Rats, Inbred Strains
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Skin / analysis*
Substances
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Cholestadienols
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Dehydrocholesterols
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vitamin D3 sulfoconjugate
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7-dehydrocholesterol-3-sulfate ester
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Cholecalciferol
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25-hydroxyvitamin D3 3-sulfate ester
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Calcifediol