Abstract
Squalene synthetase has been purified to homogeneity from yeast. It is a single polypeptide of Mr 47,000. This enzyme catalyzes the synthesis of squalene from farnesyl diphosphate via presqualene diphosphate. In the presence of reduced pyridine nucleotides, presqualene diphosphate and squalene are produced in a ratio of 6:1 from either the purified protein or the crude microsomal fraction.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Chromatography
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Electrophoresis, Polyacrylamide Gel
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Farnesyl-Diphosphate Farnesyltransferase / isolation & purification*
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Farnesyl-Diphosphate Farnesyltransferase / metabolism
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Isoelectric Focusing
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Molecular Weight
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NAD / pharmacology
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NADP / pharmacology
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Oxidoreductases / isolation & purification*
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Polyisoprenyl Phosphates / biosynthesis
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Polyisoprenyl Phosphates / metabolism
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Saccharomyces cerevisiae / enzymology*
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Sesquiterpenes
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Squalene / biosynthesis
Substances
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Polyisoprenyl Phosphates
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Sesquiterpenes
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NAD
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presqualene pyrophosphate
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NADP
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farnesyl pyrophosphate
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Squalene
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Oxidoreductases
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Farnesyl-Diphosphate Farnesyltransferase