Presence of epoxide hydrolase and glutathione S-transferase in human pulmonary alveolar macrophages

S Petruzzelli; P Bernard; P Paoletti; A Rane; C Giuntini; G M Pacifici

doi:10.1007/BF00542447

Presence of epoxide hydrolase and glutathione S-transferase in human pulmonary alveolar macrophages

Eur J Clin Pharmacol. 1988;34(4):419-21. doi: 10.1007/BF00542447.

Authors

S Petruzzelli¹, P Bernard, P Paoletti, A Rane, C Giuntini, G M Pacifici

Affiliation

¹ Institute of 2nd Medical Clinic, University of Pisa, Italy.

PMID: 3402528
DOI: 10.1007/BF00542447

Abstract

Pulmonary alveolar macrophages (PAMs) were obtained from 11 patients by bronchoalveolar lavage. Epoxide hydrolase and glutathione S-transferase in sonicated PAMs were measured using benzo(a)pyrene-4,5-oxide as the substrate. The activity of epoxide hydrolase was 0.24 +/- 0.10 nmol/min/mg protein (mean +/- SD), and of glutathione S-transferase 0.22 +/- 0.12 nmol/min/mg protein. There was a significant difference in enzyme activities in the PAMs from smokers and non-smokers. Epoxides may be metabolized in PAMs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Epoxide Hydrolases / metabolism*
Female
Glutathione Transferase / metabolism*
Humans
Macrophages / enzymology*
Male
Middle Aged
Pulmonary Alveoli / cytology
Pulmonary Alveoli / enzymology*

Substances

Glutathione Transferase
Epoxide Hydrolases