Cloning and expression of a human glucagon receptor

Biochem Biophys Res Commun. 1994 Jan 14;198(1):328-34. doi: 10.1006/bbrc.1994.1046.

Abstract

A human glucagon receptor has been cloned from human liver tissue. The 1578-bp cDNA clone encodes a protein of 477 amino acids with 82% identity to the rat glucagon receptor. The predicted secondary structure and homology to known proteins places this receptor within the superfamily of seven transmembrane domain G protein coupled receptors. Transfection of the human glucagon receptor into COS-7 cells confers upon them high affinity binding for [125I] glucagon. In membranes prepared from COS-7 cells transfected with the human glucagon receptor, the binding of [125I] glucagon is inhibited with the rank order of potency glucagon > oxyntomodulin > glucagon-like peptide 1 (7-36) amide >> glucagon-like peptide 2 = gastric inhibitory peptide = secretin.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding, Competitive
  • Cell Line
  • Cell Membrane / metabolism
  • Chlorocebus aethiops
  • Cloning, Molecular / methods
  • DNA Primers
  • DNA, Complementary / metabolism
  • Gene Expression
  • Glucagon / metabolism
  • Humans
  • Kidney
  • Kinetics
  • Liver / metabolism*
  • Molecular Sequence Data
  • Poly A / isolation & purification
  • Poly A / metabolism
  • Polymerase Chain Reaction / methods
  • RNA / isolation & purification
  • RNA / metabolism
  • RNA, Messenger
  • Rats
  • Receptors, Glucagon / biosynthesis*
  • Receptors, Glucagon / genetics
  • Receptors, Glucagon / metabolism
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Transfection

Substances

  • DNA Primers
  • DNA, Complementary
  • RNA, Messenger
  • Receptors, Glucagon
  • Recombinant Proteins
  • Poly A
  • RNA
  • Glucagon

Associated data

  • GENBANK/U03469