Active transport of benzo[a]pyrene in apical membrane vesicles from normal human intestinal epithelium

Biochim Biophys Acta. 1994 May 25;1226(2):232-6. doi: 10.1016/0925-4439(94)90034-5.

Abstract

Transport of the carcinogen benzo[a]pyrene in apical membrane vesicles (AMV) from normal human intestine, was investigated. Benzo[a]pyrene transport was found in AMV throughout the small intestine, but was greatest in colon. Evidence suggesting involvement of P-Glycoprotein (P-Gp), included (1) comparable transport of P-Gp substrate doxorubicin, (2) transport stimulation by ATP and (3) transport suppression by the P-Gp inhibitor, verapamil.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Adenosine Triphosphate / pharmacology
  • Adult
  • Aged
  • Alkaline Phosphatase / analysis
  • Aminopeptidases / analysis
  • Benzo(a)pyrene / metabolism*
  • Biological Transport, Active / drug effects
  • CD13 Antigens
  • Carrier Proteins / metabolism
  • Cell Membrane / metabolism
  • Colon / metabolism*
  • Colon / ultrastructure
  • Doxorubicin / metabolism
  • Female
  • Humans
  • Intestine, Small / metabolism*
  • Intestine, Small / ultrastructure
  • Male
  • Membrane Glycoproteins / metabolism
  • Middle Aged
  • Verapamil / pharmacology

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Carrier Proteins
  • Membrane Glycoproteins
  • Benzo(a)pyrene
  • Doxorubicin
  • Adenosine Triphosphate
  • Verapamil
  • Alkaline Phosphatase
  • Aminopeptidases
  • CD13 Antigens