Abstract
A 1,179 bp and a 1,424 bp full-length aryl sulfotransferase cDNAs were isolated from a human brain cDNA library. Their coding domains are 93% identical, each encoding a cytosolic protein of 295 amino acids. Their deduced amino acid sequences of these cDNAs are also 93% identical. The 1179 bp brain cDNA has an identical coding domain to a previously reported human liver aryl sulfotransferase cDNA but it has a different 5' noncoding sequence. Northern blot analysis using a probe specific for the 1,424 bp cDNA identified a 1500 bp band in mRNA of human liver, colon, kidney and lung. In a human hepatocellular carcinoma the same band plus an extra larger band was also recognised. An intron of the gene encoding the 1424 bp cDNA was also identified.
MeSH terms
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Amino Acid Sequence
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Arylsulfotransferase / biosynthesis*
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Arylsulfotransferase / genetics
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Base Sequence
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Blotting, Northern
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Brain / enzymology*
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Cloning, Molecular
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DNA / analysis*
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DNA / genetics
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Electrophoresis, Agar Gel
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Gene Library
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Humans
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Introns
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Isoenzymes / biosynthesis*
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Isoenzymes / genetics
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Polymerase Chain Reaction
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RNA, Messenger / isolation & purification
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RNA, Messenger / metabolism*
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
Substances
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Isoenzymes
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Oligodeoxyribonucleotides
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RNA, Messenger
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DNA
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Arylsulfotransferase
Associated data
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GENBANK/D37827
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GENBANK/L16877
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GENBANK/L16878
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GENBANK/L19108
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GENBANK/L19109
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GENBANK/L19111
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GENBANK/L19112
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GENBANK/L19955
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GENBANK/L19956
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GENBANK/L19957