Identification of two human brain aryl sulfotransferase cDNAs

Biochem Biophys Res Commun. 1993 Aug 31;195(1):120-7. doi: 10.1006/bbrc.1993.2018.

Abstract

A 1,179 bp and a 1,424 bp full-length aryl sulfotransferase cDNAs were isolated from a human brain cDNA library. Their coding domains are 93% identical, each encoding a cytosolic protein of 295 amino acids. Their deduced amino acid sequences of these cDNAs are also 93% identical. The 1179 bp brain cDNA has an identical coding domain to a previously reported human liver aryl sulfotransferase cDNA but it has a different 5' noncoding sequence. Northern blot analysis using a probe specific for the 1,424 bp cDNA identified a 1500 bp band in mRNA of human liver, colon, kidney and lung. In a human hepatocellular carcinoma the same band plus an extra larger band was also recognised. An intron of the gene encoding the 1424 bp cDNA was also identified.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Arylsulfotransferase / biosynthesis*
  • Arylsulfotransferase / genetics
  • Base Sequence
  • Blotting, Northern
  • Brain / enzymology*
  • Cloning, Molecular
  • DNA / analysis*
  • DNA / genetics
  • Electrophoresis, Agar Gel
  • Gene Library
  • Humans
  • Introns
  • Isoenzymes / biosynthesis*
  • Isoenzymes / genetics
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction
  • RNA, Messenger / isolation & purification
  • RNA, Messenger / metabolism*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid

Substances

  • Isoenzymes
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • DNA
  • Arylsulfotransferase

Associated data

  • GENBANK/D37827
  • GENBANK/L16877
  • GENBANK/L16878
  • GENBANK/L19108
  • GENBANK/L19109
  • GENBANK/L19111
  • GENBANK/L19112
  • GENBANK/L19955
  • GENBANK/L19956
  • GENBANK/L19957