Abstract
Genomic DNA clones for nicotinamide N-methyltransferase (NNMT), an enzyme that catalyzes drug and xenobiotic metabolism, were isolated from a human chromosome 11-specific DNA library. Study of one of those clones, when combined with PCR-based experiments performed with human genomic DNA, made it possible to determine the structure of the human NNMT gene. The gene was approximately 16.5 kb in length and consisted of 3 exons and 2 introns. Transcription initiation for the NNMT gene occurred 105-109 nucleotides 5'-upstream from the cDNA translation initiation codon on the basis of the results of both primer extension and 5'-rapid amplification of cDNA ends. NNMT mapped to chromosome band 11q23.1 by fluorescence in situ hybridization.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cattle
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Chromosome Mapping
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Chromosomes, Human, Pair 11*
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Cloning, Molecular
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Codon
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DNA Primers
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Exons
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Gene Library
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Humans
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In Situ Hybridization, Fluorescence
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Introns
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Liver / enzymology*
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Methyltransferases / biosynthesis
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Methyltransferases / genetics*
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Mice
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Molecular Sequence Data
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Nicotinamide N-Methyltransferase
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Oligonucleotides, Antisense
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Peptide Chain Initiation, Translational
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Polymerase Chain Reaction
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Rats
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Recombinant Proteins / biosynthesis
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Regulatory Sequences, Nucleic Acid*
Substances
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Codon
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DNA Primers
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Oligonucleotides, Antisense
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Recombinant Proteins
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Methyltransferases
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NNMT protein, human
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Nicotinamide N-Methyltransferase
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Nnmt protein, mouse
Associated data
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GENBANK/U20970
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GENBANK/U20971