Multidrug resistance-associated protein (MRP) gene expression in human lung cancer

F Narasaki; I Matsuo; N Ikuno; M Fukuda; H Soda; M Oka

Multidrug resistance-associated protein (MRP) gene expression in human lung cancer

Anticancer Res. 1996 Jul-Aug;16(4A):2079-82.

Authors

F Narasaki¹, I Matsuo, N Ikuno, M Fukuda, H Soda, M Oka

Affiliation

¹ Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.

PMID: 8712746

Abstract

Multidrug resistance-associated protein (MRP) is a 190 kD transmembrane protein and a potentially important drug-transporter protein in human cancers. While the MRP gene is expressed in normal cells and tissues, the expression in solid tumors is not sufficiently determined. MRP and mdr1 mRNA expressions were examined in normal lung parenchyma and in tumor tissues from six small cell lung cancer (SCLC) patients who had received preoperative chemotherapy and eleven nonsmall cell lung cancer (NSCLC) patients. The reverse transcriptase polymerase chain reaction was used. Normal lung tissues and all SCLCs expressed abundant levels of MRP mRNA, while the NSCLCs expressed a wide range of levels from low to high. Most tumor tissues coexpressed both MRP and mdr1, but the levels of mdrl expression was low except in two SCLCs and one NSCLC. MRP is more likely than mdr1 to be one of the clinical multidrug resistance mechanisms found in lung cancer.

Publication types

Comparative Study

MeSH terms

ATP Binding Cassette Transporter, Subfamily B, Member 1 / analysis
ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis*
ATP-Binding Cassette Transporters / analysis
ATP-Binding Cassette Transporters / biosynthesis*
Breast Neoplasms
Carcinoma, Non-Small-Cell Lung / drug therapy
Carcinoma, Non-Small-Cell Lung / metabolism*
Carcinoma, Non-Small-Cell Lung / pathology
Carcinoma, Non-Small-Cell Lung / surgery
Carcinoma, Small Cell / drug therapy
Carcinoma, Small Cell / metabolism*
Carcinoma, Small Cell / pathology
Carcinoma, Small Cell / surgery
Cell Line
Doxorubicin / toxicity
Drug Resistance, Multiple*
Etoposide / toxicity
Female
Gene Expression
HL-60 Cells
Humans
Lung / metabolism
Lung Neoplasms / drug therapy
Lung Neoplasms / metabolism*
Lung Neoplasms / pathology
Lung Neoplasms / surgery
Multidrug Resistance-Associated Proteins
Polymerase Chain Reaction
Reference Values
Vincristine / toxicity

Substances

ATP Binding Cassette Transporter, Subfamily B, Member 1
ATP-Binding Cassette Transporters
Multidrug Resistance-Associated Proteins
Vincristine
Etoposide
Doxorubicin