Isolation and characterization of a bovine gene encoding phenol sulfotransferase

Gene. 1996 Oct 3;174(2):221-4. doi: 10.1016/0378-1119(96)00083-2.

Abstract

Phenol sulfotransferases (PST) esterify hydroxylated aromatic compounds with sulfate, and therefore play a role in the metabolism of xenobiotics. In this investigation, a bovine PST gene of 2372 bp was amplified from genomic DNA. Sequence overlap with the cognate cDNA revealed seven exons, with all introns containing GT/AG splicing donor/acceptor site. This product was subcloned into an SV40-based expression vector and transfected into WI-26 human lung fibroblasts. Immunoblot analyses revealed production of the anticipated 32-kDa protein, and the active enzyme displayed steady-state kinetic properties consistent with the enzyme characterized in bovine lung (apparent Km = 6.5 microM for 2-naphthol).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arylsulfotransferase / genetics*
  • Base Sequence
  • Cattle
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Recombinant
  • Exons
  • Gene Expression Regulation
  • Genetic Vectors
  • Humans
  • Immunoblotting
  • Introns
  • Molecular Sequence Data
  • Transfection

Substances

  • DNA, Recombinant
  • Arylsulfotransferase

Associated data

  • GENBANK/U34753